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Typical Testing Data/Standard Curve (for reference only)

Chicken T3 (Triiodothyronine) ELISA Kit | T3 elisa kit

Chicken T3 (Triiodothyronine) ELISA Kit

Gene Names
THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
Reactivity
Chicken
Synonyms
T3 (Triiodothyronine); Chicken T3 (Triiodothyronine) ELISA Kit; T3 elisa kit
Ordering
For Research Use Only!
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of T. No significant crossreactivity or interference between T and analogues is observed.
Sequence Length
75
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Competitive
Detection Range
0.313-20ng/ml
Sensitivity
<0.188ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for T3 elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with T. During the reaction, T in the sample or standard competes with a fixed amount of T on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of T in the samples is then determined by comparing the O D of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
46,813 Da
NCBI Official Full Name
triiodothyronine receptor, partial
NCBI Official Synonym Full Names
thyroid hormone receptor alpha
NCBI Official Symbol
THRA
NCBI Official Synonym Symbols
AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
NCBI Protein Information
thyroid hormone receptor alpha
UniProt Protein Name
Thyroid hormone receptor alpha
UniProt Gene Name
THRA
UniProt Synonym Gene Names
EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Isoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

Research Articles on T3

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Product Notes

The Chicken T3 thra (Catalog #AAA7607169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7607169 ELISA Kit recognizes Chicken T3. It is sometimes possible for the material contained within the vial of "T3 (Triiodothyronine), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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