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Typical Testing Data/Standard Curve (for reference only)

Porcine Triiodothyronine (T3) ELISA Kit | T3 elisa kit

Porcine Triiodothyronine (T3) ELISA Kit

Gene Names
THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
Reactivity
Porcine
Synonyms
Triiodothyronine (T3); Porcine Triiodothyronine (T3) ELISA Kit; T3 elisa kit
Ordering
For Research Use Only!
Reactivity
Porcine
Specificity
No cross-reaction with other factors.
Sequence Length
490
Samples
Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant T3 concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for T3 elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine T3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,465 Da
NCBI Official Full Name
thyroid hormone receptor alpha isoform 2
NCBI Official Synonym Full Names
thyroid hormone receptor, alpha
NCBI Official Symbol
THRA
NCBI Official Synonym Symbols
AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
NCBI Protein Information
thyroid hormone receptor alpha; EAR-7; c-erbA-alpha; ERBA-related 7; V-erbA-related protein 7; triiodothyronine receptor; nuclear receptor subfamily 1 group A member 1; thyroid normone nuclear receptor alpha variant 1; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
UniProt Protein Name
Thyroid hormone receptor alpha
UniProt Gene Name
THRA
UniProt Synonym Gene Names
EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
UniProt Entry Name
THA_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.

Protein type: Transcription factor; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein binding; zinc ion binding; chromatin DNA binding; TATA-binding protein binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; ossification; cytoplasmic sequestering of transcription factor; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation

Disease: Hypothyroidism, Congenital, Nongoitrous, 6

Research Articles on T3

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Product Notes

The Porcine T3 thra (Catalog #AAA269472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA269472 ELISA Kit recognizes Porcine T3. It is sometimes possible for the material contained within the vial of "Triiodothyronine (T3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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