Rabbit anti-Human TC10 Polyclonal Antibody | anti-TC10 antibody

TC10, ID (RHOQ, ARHQ, RASL7A, TC10, Rho-related GTP-binding protein RhoQ, Ras-like protein TC10, Ras-like protein family member 7A) (AP)

Cat. Num. AAA6354271

• Gene Names: RHOQ; ARHQ; TC10; TC10A; RASL7A; HEL-S-42
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TC10; Polyclonal Antibody; ID (RHOQ; ARHQ; RASL7A; Rho-related GTP-binding protein RhoQ; Ras-like protein TC10; Ras-like protein family member 7A) (AP); RHOQ; Ras-like protein family member 7A; anti-TC10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TC10 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human TC10.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TC10 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TC10 antibody detected the TC10 protein (arrow).)

Immunohistochemistry (IHC)

(TC10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TC10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-TC10 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 50263042
• NCBI GeneID: 23433
• NCBI Accession #: NP_036381.2
• NCBI GenBank Nucleotide #: NM_012249.3
• UniProt Accession #: P17081; Q0VGN1; Q52LS8; Q53SJ1; Q6NS39; Q6P146; Q7Z480; D6W5A6
• Molecular Weight: 22,659 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoQ
• NCBI Official Synonym Full Names: ras homolog family member Q
• NCBI Official Symbol: RHOQ
• NCBI Official Synonym Symbols: ARHQ; TC10; TC10A; RASL7A; HEL-S-42
• NCBI Protein Information: rho-related GTP-binding protein RhoQ; RAS-like, family 7, member A; epididymis secretory protein Li 42; ras homolog gene family, member Q; ras-like protein TC10; ras-like protein family member 7A
• UniProt Protein Name: Rho-related GTP-binding protein RhoQ
• UniProt Gene Name: RHOQ
• UniProt Synonym Gene Names: ARHQ; RASL7A; TC10
• UniProt Entry Name: RHOQ_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein is an important signalling protein for sarcomere assembly and has been shown to play a significant role in the exocytosis of the solute carrier family 2, facilitated glucose transporter member 4 and other proteins, possibly acting as the signal that turns on the membrane fusion machinery. Three related pseudogene have been identified on chromosomes 2 and 14. [provided by RefSeq, Aug 2011]

Uniprot Description

RHOQ: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rho; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: plasma membrane; actin filament; cytosol; lipid raft

Molecular Function: GTPase activity; GTP binding; GBD domain binding; profilin binding

Biological Process: positive regulation of filopodium formation; regulation of cell shape; positive regulation of glucose import; regulation of small GTPase mediated signal transduction; cellular response to insulin stimulus; metabolic process; regulation of actin cytoskeleton organization and biogenesis; small GTPase mediated signal transduction; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cortical actin cytoskeleton organization and biogenesis