Chicken Interleukin 17 (IL17) ELISA Kit | IL17 elisa kit

Chicken Interleukin 17 (IL17) ELISA Kit

Cat. Num. AAA2020130

• Gene Names: IL17A; IL17; CTLA8; IL-17; IL-17A
• Reactivity: Chicken
• Synonyms: Interleukin 17 (IL17); Chicken Interleukin 17 (IL17) ELISA Kit; IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17 elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17). ; No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.
• Sequence Length: 9

• Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 15.63-1000pg/mL
• Sensitivity: Typically less than 5.6pg/mL.
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively. ; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. ; CV(%) = SD/meanX100 ; Intra-Assay: CV<10% ; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards;; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;; 4. Aspirate and wash 3 times;; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;; 6. Aspirate and wash 5 times;; 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;; 8. Add 50uL Stop Solution. Read at 450nm immediately.
• Preparation and Storage: For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.; ; For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.; ; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL17 elisa kit

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17 (IL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 17 (IL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55139064
• NCBI GeneID: 3605
• Molecular Weight: 933 Da
• NCBI Official Full Name: interleukin-17, partial
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: IL17A
• NCBI Official Synonym Symbols: IL17; CTLA8; IL-17; IL-17A
• NCBI Protein Information: interleukin-17A
• UniProt Protein Name: Interleukin-17
• UniProt Gene Name: IL17
• UniProt Entry Name: Q5QEX9_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: cell death; cell-cell signaling; apoptosis; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response