Human MVP ELISA Kit | MVP elisa kit

Human MVP (Major Vault Protein) ELISA Kit

Cat. Num. AAA2500741

• Gene Names: MVP; LRP; VAULT1
• Reactivity: Human
• Synonyms: MVP; Human MVP (Major Vault Protein) ELISA Kit; MVP elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human MUC5B in samples. No Significant cross-reactivity or interference between Human MUC5B and analogues was observed.
• Sequence Length: 893

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.63-40 ng/mL
• Sensitivity: 0.38 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MUC5B were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MUC5B were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MVP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MUC5B concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC5B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MUC5B. You can calculate the concentration of Human MUC5B in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19913412
• NCBI GeneID: 9961
• NCBI Accession #: NP_005106.2
• NCBI GenBank Nucleotide #: NM_005115.4
• UniProt Accession #: Q14764; Q96BG4; Q9BPW6; Q9BQT1; Q9UBD1
• Molecular Weight: 99,327 Da
• NCBI Official Full Name: major vault protein isoform 1
• NCBI Official Synonym Full Names: major vault protein
• NCBI Official Symbol: MVP
• NCBI Official Synonym Symbols: LRP; VAULT1
• NCBI Protein Information: major vault protein; lung resistance-related protein
• UniProt Protein Name: Major vault protein
• Protein Family: Hyperpolarization-activated voltage-gated potassium channel
• UniProt Gene Name: MVP
• UniProt Synonym Gene Names: LRP; MVP
• UniProt Entry Name: MVP_HUMAN

NCBI Description

This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

MVP: Required for normal vault structure. Vaults are multi- subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo- cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoskeleton; membrane; perinuclear region of cytoplasm; cytoplasm; nuclear pore; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; protein kinase binding; protein phosphatase binding

Biological Process: cell proliferation; protein transport; mRNA transport; negative regulation of protein amino acid autophosphorylation