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Immunofluorescence (IF) (Immunofluorescence analysis of HUVEC cells, using MED24 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit MED24 Polyclonal Antibody | anti-MED24 antibody

MED24 Antibody

Gene Names
MED24; MED5; CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
MED24; Polyclonal Antibody; MED24 Antibody; Mediator of RNA polymerase II transcription subunit 24; Mediator complex subunit 24; hTRAP100; Trap100; Thyroid hormone receptor-associated protein 4; Vitamin D3 receptor-interacting protein complex component DRIP100; Activator-recruited cofactor 100 kDa; anti-MED24 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
MED24 Antibody detects endogenous levels of total MED24 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
976
Applicable Applications for anti-MED24 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human MED24.
Immunogen Range
801-850
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HUVEC cells, using MED24 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HUVEC cells, using MED24 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from 293 cells, using MED24 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from 293 cells, using MED24 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
110 kDa
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 24 isoform 2
NCBI Official Synonym Full Names
mediator complex subunit 24
NCBI Official Symbol
MED24
NCBI Official Synonym Symbols
MED5; CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 24
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 24
UniProt Gene Name
MED24
UniProt Synonym Gene Names
ARC100; CRSP4; DRIP100; KIAA0130; THRAP4; TRAP100; ARC100; CRSP complex subunit 4; Trap100; hTRAP100; DRIP100

NCBI Description

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Research Articles on MED24

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Product Notes

The MED24 med24 (Catalog #AAA9504862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED24 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MED24 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the MED24 med24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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