ULBP1 recombinant protein

ULBP1, human recombinant

Cat. Num. AAA845314

• Gene Names: ULBP1; RAET1I
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: ULBP1; human recombinant; NKG2D ligand 1; UL16 binding protein 1; RAET1I; N2DL-1; N2DL1; ULBP1 recombinant protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 30% glycerol and 1 mM DTT. ; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHM GWVDT HCLCYDFIIT PKSRPEPQWC EVQGLVDERP FLHYDCVNHK AKAFASLGKK VNVTKTWEEQ TETLRDVVDF LKGQLLDIQV ENLIPIEPLT LQARMSCEHE AHGHGRGSWQ FLFNGQKFLL FDSNNRKWTA LHPGAKKMTE KWEKNRDVTM FFQKISLGDC KMWLEEFLMY WEQMLDPTKP PSLAPG
• Sequence Length: 244

• Applicable Applications for ULBP1 recombinant protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for ULBP1 recombinant protein

Background: ULBP1 is ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. In CMV infected cells, ULBP1 interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. Recombinant human ULBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for ULBP1 recombinant protein

Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 13376826
• NCBI GeneID: 80329
• NCBI Accession #: NP_079494.1
• NCBI GenBank Nucleotide #: NM_025218.2
• UniProt Accession #: Q9BZM6; Q5VY81; Q8IZW3; Q8IZX6
• Molecular Weight: 25 kDa (216 aa, 26-216 aa + His Tag), confirmed by MALDI-TOF.
• NCBI Official Full Name: NKG2D ligand 1
• NCBI Official Synonym Full Names: UL16 binding protein 1
• NCBI Official Symbol: ULBP1
• NCBI Official Synonym Symbols: RAET1I
• NCBI Protein Information: NKG2D ligand 1
• UniProt Protein Name: NKG2D ligand 1
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP1
• UniProt Synonym Gene Names: N2DL1; RAET1I; N2DL-1; NKG2DL1
• UniProt Entry Name: N2DL1_HUMAN

Uniprot Description

ULBP1: Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor; Apoptosis; Cell surface

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: anchored to plasma membrane; endoplasmic reticulum; plasma membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; natural killer cell activation