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Typical Standard Curve/Testing Data

Chicken Cholecystokinin 8 (CCK8) ELISA Kit | CCK8 elisa kit

Chicken Cholecystokinin 8 (CCK8) ELISA Kit

Reactivity
Chicken
Synonyms
Cholecystokinin 8 (CCK8); Chicken Cholecystokinin 8 (CCK8) ELISA Kit; CCK8 elisa kit
Ordering
For Research Use Only!
Reactivity
Chicken
Specificity
No cross-reaction with other factors.
Sequence Length
115
Samples
Serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
1000 pg/ml-15.6 pg/ml
Sensitivity
Up to 5 pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Typical Standard Curve/Testing Data
Related Product Information for CCK8 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken CCK8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
885
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,669 Da
NCBI Official Full Name
cholecystokinin preproprotein
NCBI Official Synonym Full Names
cholecystokinin
NCBI Official Symbol
CCK
NCBI Protein Information
cholecystokinin; prepro-cholecystokinin; cholecystokinin triacontatriapeptide
UniProt Protein Name
Cholecystokinin
UniProt Gene Name
CCK
UniProt Synonym Gene Names
CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5
UniProt Entry Name
CCKN_HUMAN

NCBI Description

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation; Hormone

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: axon hillock; extracellular space; axon; dendrite; extracellular region; terminal button; perikaryon

Molecular Function: neuropeptide hormone activity; protein binding; hormone activity

Biological Process: caspase activation; positive regulation of protein oligomerization; release of cytochrome c from mitochondria; positive regulation of peptidyl-tyrosine phosphorylation; behavioral fear response; axonogenesis; positive regulation of mitochondrial depolarization; eating behavior; protein kinase C activation; positive regulation of cell proliferation; neuron migration; regulation of sensory perception of pain; signal transduction; negative regulation of appetite

Research Articles on CCK8

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Product Notes

The Chicken CCK8 cck (Catalog #AAA264630) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA264630 ELISA Kit recognizes Chicken CCK8. It is sometimes possible for the material contained within the vial of "Cholecystokinin 8 (CCK8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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