Chicken BLVRB ELISA Kit | BLVRB elisa kit

Chicken BLVRB (Biliverdin Reductase B) ELISA Kit

Cat. Num. AAA2502415

• Reactivity: Chicken
• Synonyms: BLVRB; Chicken BLVRB (Biliverdin Reductase B) ELISA Kit; BLVRB elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken BLVRB. No significant cross-reactivity or interference between Chicken BLVRB and analogues was observed.
• Sequence Length: 206

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BLVRB elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken BLVRB concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BLVRB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BLVRB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BLVRB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BLVRB. You can calculate the concentration of BLVRB in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13879286
• NCBI GeneID: 233016
• UniProt Accession #: Q923D2
• Molecular Weight: 22,197 Da
• NCBI Official Full Name: Blvrb protein
• NCBI Official Synonym Full Names: biliverdin reductase B (flavin reductase (NADPH))
• NCBI Official Symbol: Blvrb
• NCBI Protein Information: flavin reductase (NADPH); flavin reductase (NADPH); BVR-B; FLR; FR; NADPH-dependent diaphorase; NADPH-flavin reductase; biliverdin-IX beta-reductase
• UniProt Protein Name: Flavin reductase (NADPH)
• UniProt Gene Name: Blvrb
• UniProt Synonym Gene Names: FR; BVR-B; FLR
• UniProt Entry Name: BLVRB_MOUSE

Uniprot Description

BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Protein type: EC 1.5.1.30; Oxidoreductase; EC 1.3.1.24; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: flavin reductase activity; oxidoreductase activity; biliverdin reductase activity

Biological Process: heme catabolic process