Typical Testing Data/Standard Curve (for reference only)
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Human Biliverdin Reductase B (BLVRB) ELISA Kit | BLVRB elisa kit
Human Biliverdin Reductase B (BLVRB) ELISA Kit
Gene Names
BLVRB; FLR; BVRB; SDR43U1; HEL-S-10
Reactivity
Human
Synonyms
Biliverdin Reductase B (BLVRB); Human Biliverdin Reductase B (BLVRB) ELISA Kit; FLR; SDR43U1; Flavin Reductase; NADPH; Short Chain Dehydrogenase/Reductase Family 43U; Member 1; Biliverdin-IX beta-reductase; Green heme-binding protein; BLVRB elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of BLVRB. No significant cross-reactivity or interference between BLVRB and analogues was observed.
Sequence Length
206
Samples
Human tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.122ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BLVRB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BLVRB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for BLVRB elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BLVRB in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to BLVRB. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to BLVRB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BLVRB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BLVRB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to BLVRB. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to BLVRB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BLVRB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BLVRB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for BLVRB elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,119 Da
NCBI Official Full Name
Biliverdin reductase B (flavin reductase (NADPH))
NCBI Official Synonym Full Names
biliverdin reductase B
NCBI Official Symbol
BLVRB
NCBI Official Synonym Symbols
FLR; BVRB; SDR43U1; HEL-S-10
NCBI Protein Information
flavin reductase (NADPH)
UniProt Protein Name
Flavin reductase (NADPH)
UniProt Gene Name
BLVRB
UniProt Synonym Gene Names
FLR; FR; BVR-B; GHBP; FLR
UniProt Entry Name
BLVRB_HUMAN
NCBI Description
The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24).[supplied by OMIM, Jul 2009]
Uniprot Description
BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.
Protein type: EC 1.5.1.30; Oxidoreductase; EC 1.3.1.24; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll
Chromosomal Location of Human Ortholog: 19q13.1-q13.2
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol
Molecular Function: flavin reductase activity; biliverdin reductase activity
Biological Process: heme catabolic process; porphyrin metabolic process
Research Articles on BLVRB
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Product Notes
The Human BLVRB blvrb (Catalog #AAA2019416) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019416 ELISA Kit recognizes Human BLVRB. It is sometimes possible for the material contained within the vial of "Biliverdin Reductase B (BLVRB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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