Chicken Aryl Hydrocarbon Receptor (AhR) ELISA Kit | AhR elisa kit

Chicken Aryl Hydrocarbon Receptor (AhR) ELISA Kit

Cat. Num. AAA262394

• Gene Names: AHR; bHLHe76
• Reactivity: Chicken
• Synonyms: Aryl Hydrocarbon Receptor (AhR); Chicken Aryl Hydrocarbon Receptor (AhR) ELISA Kit; AhR elisa kit

• Reactivity: Chicken
• Specificity: No cross-reaction with other factors.
• Sequence Length: 848

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Qualitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AhR elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken AhR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502003
• NCBI GeneID: 196
• NCBI Accession #: NP_001612.1
• NCBI GenBank Nucleotide #: NM_001621.4
• Molecular Weight: 96,147 Da
• NCBI Official Full Name: aryl hydrocarbon receptor
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor
• NCBI Official Symbol: AHR
• NCBI Official Synonym Symbols: bHLHe76
• NCBI Protein Information: aryl hydrocarbon receptor; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
• UniProt Protein Name: Aryl hydrocarbon receptor
• Protein Family: Aldehyde reductase
• UniProt Gene Name: AHR
• UniProt Synonym Gene Names: BHLHE76; Ah receptor; AhR; bHLHe76
• UniProt Entry Name: AHR_HUMAN

NCBI Description

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq, Jul 2008]

Uniprot Description

AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.

Protein type: Nuclear receptor; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; signal transducer activity; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; Hsp90 protein binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; prostate gland development; blood vessel development; intracellular receptor-mediated signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; response to toxin; negative regulation of transcription from RNA polymerase II promoter; cell cycle; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of transcription, DNA-dependent; regulation of gene expression; xenobiotic metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of B cell proliferation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly