Mouse Aryl hydrocarbon receptor ELISA Kit | Ahr elisa kit

Mouse Aryl hydrocarbon receptor ELISA Kit

Cat. Num. AAA764611

• Gene Names: AHR; bHLHe76
• Reactivity: Mouse
• Synonyms: Aryl hydrocarbon receptor; Mouse Aryl hydrocarbon receptor ELISA Kit; Ahr/ (Aryl hydrocarbon receptor)/BHLHE76/Ah receptor/AH-receptor/aromatic hydrocarbon receptor/Class E basic helix-loop-helix protein 76; Ahr elisa kit

• Reactivity: Mouse
• Sequence Length: 848

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.391-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ahr elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ahr antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ahr antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ahr amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ahr can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502003
• NCBI GeneID: 196
• NCBI Accession #: NP_001612.1
• NCBI GenBank Nucleotide #: NM_001621.4
• Molecular Weight: 96,147 Da
• NCBI Official Full Name: aryl hydrocarbon receptor
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor
• NCBI Official Symbol: AHR
• NCBI Official Synonym Symbols: bHLHe76
• NCBI Protein Information: aryl hydrocarbon receptor
• UniProt Protein Name: Aryl hydrocarbon receptor
• Protein Family: Aldehyde reductase
• UniProt Gene Name: AHR
• UniProt Synonym Gene Names: BHLHE76; Ah receptor; AhR; bHLHe76
• UniProt Entry Name: AHR_HUMAN

NCBI Description

The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]

Uniprot Description

AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: cytoplasm; nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; Hsp90 protein binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: apoptosis; blood vessel development; cell cycle; circadian regulation of gene expression; intracellular receptor-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; ovarian follicle development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly; prostate gland development; regulation of B cell proliferation; regulation of gene expression; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to estradiol stimulus; response to toxin; response to xenobiotic stimulus; transcription from RNA polymerase II promoter; xenobiotic metabolic process