Chromogranin B (CHGB) Recombinant Protein | CHGB recombinant protein

Recombinant Chromogranin B (CHGB)

Cat. Num. AAA2011279

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Chromogranin B (CHGB); Recombinant Chromogranin B (CHGB); CHGB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-V LSNALSKSNA PPITPECRQV LKKGGKEVKD EEKGANENTR FEVRLLRDPA DTSETQRPSG GREGAEAPSE DTQGPPGADVEGGGHSREGA GKPRGGPYSS DNPVAKEGKT RHSEKSEGQD REEEEGEKYQ KRERGEEGSE ERHLQEPGET QTAFLNQGNR ATAKKKEEFE SRYDAHSAGG PAKTHSRERS SQESGEETGSQDAAPGEPES PPEGQEAPEE SQEDASLEVD KRRWRPRHHH GRSRPDRAPQ EGSPPPEERG HPREESEESV LGLASPGGQR TRHPTH
• Sequence Length: 668

• Applicable Applications for CHGB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Val40~His326 (Accession # Q9GLG4) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 25453269
• UniProt Accession #: Q9GLG4
• Molecular Weight: 58.4kDa
• NCBI Official Full Name: Secretogranin-1
• UniProt Protein Name: Secretogranin-1
• Protein Family: Secretogranin
• UniProt Gene Name: CHGB
• UniProt Synonym Gene Names: CgB; SgI
• UniProt Entry Name: SCG1_PIG

Uniprot Description

Function: Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.

Subcellular location: Secreted. Note: Neuroendocrine and endocrine secretory granules.

Post-translational modification: Peptide SR-17 exists in both monophosphorylated and diphosphorylated forms. Ref.1

Sequence similarities: Belongs to the chromogranin/secretogranin protein family.

Caution: Ref.1 reported the oxidation of Met-651 to sulfoxide. This is most probably an artifact of isolation.

Mass spectrometry: Molecular mass is 2034.04 Da from positions 606 - 622. Determined by ESI. With 1 phosphate group. Ref.1Molecular mass is 2115.42 Da from positions 606 - 622. Determined by ESI. With 2 phosphate groups. Ref.1Molecular mass is 3815.56 Da from positions 623 - 656. Determined by ESI. Ref.1Molecular mass is 1305.34 Da from positions 657 - 667. Determined by ESI. Ref.1