Rabbit anti-Human GCNT2 Polyclonal Antibody | anti-GCNT2 antibody

GCNT2, ID (GCNT2, GCNT5, II, NACGT1, N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform A, I-branching enzyme, IGNT) (PE)

Cat. Num. AAA6301697

• Gene Names: GCNT2; II; CCAT; IGNT; ULG3; GCNT5; GCNT2C; NACGT1; NAGCT1; CTRCT13; bA421M1.1; bA360O19.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GCNT2; Polyclonal Antibody; ID (GCNT2; GCNT5; II; NACGT1; N-acetyllactosaminide beta-1; 6-N-acetylglucosaminyl-transferase; isoform A; I-branching enzyme; IGNT) (PE); IGNT; anti-GCNT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GCNT2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GCNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the Central region of human GCNT2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GCNT2 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GCNT2 Antibody detected the GCNT2 protein (arrow).)

Related Product Information for anti-GCNT2 antibody

This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Categories/Family for anti-GCNT2 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Twu, Y.C., et al. Blood 110(13):4526-4534(2007). Wang, L., et al. Biochem. Biophys. Res. Commun. 331(4):958-963(2005). Pras, E., et al. Invest. Ophthalmol. Vis. Sci. 45(6):1940-1945(2004). Inaba, N., et al. Blood 101(7):2870-2876(2003). Yu, L.C., et al. Blood 101(6):2081-2088(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 21717810
• NCBI GeneID: 2651
• NCBI Accession #: NP_663624.1
• NCBI GenBank Nucleotide #: NM_145649.4
• UniProt Accession #: Q8N0V5; Q06430; Q8NFS9
• Molecular Weight: 45,873 Da
• NCBI Official Full Name: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase isoform A
• NCBI Official Synonym Full Names: glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
• NCBI Official Symbol: GCNT2
• NCBI Official Synonym Symbols: II; CCAT; IGNT; ULG3; GCNT5; GCNT2C; NACGT1; NAGCT1; CTRCT13; bA421M1.1; bA360O19.2
• NCBI Protein Information: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
• UniProt Protein Name: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform A
• Protein Family: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
• UniProt Gene Name: GCNT2
• UniProt Synonym Gene Names: GCNT5; II; NACGT1; N-acetylglucosaminyltransferase
• UniProt Entry Name: GNT2A_HUMAN

NCBI Description

This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

GCNT2A: Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells. The expression of the blood group I antigen in erythrocytes is determined by isoform C. Belongs to the glycosyltransferase 14 family. 3 isoforms of the human protein are produced by alternative splicing

Protein type: EC 2.4.1.150

Chromosomal Location of Human Ortholog: 6p24.2

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: glycosaminoglycan biosynthetic process; positive regulation of protein kinase B signaling cascade; positive regulation of heterotypic cell-cell adhesion; multicellular organismal development; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; protein amino acid glycosylation; positive regulation of cell migration

Disease: Blood Group, I System