Chromogranin B (CHGB) Recombinant Protein | CHGB recombinant protein

Recombinant Chromogranin B (CHGB)

Cat. Num. AAA2011563

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Chromogranin B (CHGB); Recombinant Chromogranin B (CHGB); CHGB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-LE EGAEYGEEVR RHSAAQAPGD LQGARFGGRG RGEHQALRRP SEESLEQENK RHGLSPDLNM AQGYSEESEE ERGPAPGPSY RARGGEAAAY STLGQTDEKR FLGETHHRVQ ESQRDKARRR LPGELRNYLD YGEEKGEEAA RGKWQPQGDP RDADENREEA RLRGKQYAPH HITE
• Sequence Length: 646

• Applicable Applications for CHGB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Leu309~Glu484 (Accession # P23389) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 30794308
• NCBI GeneID: 281071
• NCBI Accession #: NP_851349.1
• NCBI GenBank Nucleotide #: NM_181006.2
• UniProt Accession #: P23389; O02707
• Molecular Weight: 51.8kDa
• NCBI Official Full Name: secretogranin-1
• NCBI Official Symbol: CHGB
• NCBI Protein Information: secretogranin-1; cgB; sgI; chromogranin-B; secretogranin I
• UniProt Protein Name: Secretogranin-1
• Protein Family: Secretogranin
• UniProt Gene Name: CHGB
• UniProt Synonym Gene Names: CgB; SgI
• UniProt Entry Name: SCG1_BOVIN

Uniprot Description

Function: Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides. The 16 pairs of basic AA distributed throughout its sequence may be used as proteolytic cleavage sites.Secretolytin has antibacterial activity.

Subcellular location: Secretogranin-1: Cytoplasmic vesicle › secretory vesicle membrane; Peripheral membrane protein; Lumenal side. Note: Neuroendocrine and endocrine secretory granules. Found to be membrane bound inside the secretory vesicles. The N-terminal region exhibits pH-dependent membrane-binding activity, binds to the membrane at intravesicular pH (5.5) and freed when the pH is near to physiological pH. Ref.4Peptide BAM-1745: Secreted Ref.4. Secretolytin: Secreted Ref.4.

Post-translational modification: O-glycosylated by the trisaccharide, GalNAc-Gal-NeuAc, on 2 sites in the N-terminal. May be glycated. Ref.8Extensively phosphorylated. Ref.8Differentially processed on numerous sites throughout the sequence depending on tissue type.

Sequence similarities: Belongs to the chromogranin/secretogranin protein family.

Mass spectrometry: Molecular mass is 2053 Da from positions 161 - 178. Determined by MALDI. Monophosphorylated. Ref.8Molecular mass is 2338 Da from positions 276 - 299. Determined by MALDI. Monophosphorylated. Ref.8Molecular mass is 6952 Da from positions 300 - 360. Determined by MALDI. Monophosphorylated and monosulfated. Ref.8Molecular mass is 5304 Da from positions 361 - 409. Determined by MALDI. Monophosphorylated. Ref.8Molecular mass is 2417 Da from positions 427 - 445. Determined by MALDI. Monosulfated. Ref.8Molecular mass is 1223 Da from positions 476 - 485. Determined by MALDI. Pyroglutamate. Ref.8Molecular mass is 3933 Da from positions 486 - 516. Determined by MALDI. Diphosphorylated. Ref.8Molecular mass is 1919 Da from positions 530 - 542. Determined by MALDI. Monosulfated. Ref.8Molecular mass is 1746 Da from positions 567 - 580. Determined by MALDI. Pyroglutamate. Ref.8Molecular mass is 1746 Da from positions 567 - 580. Determined by FAB. Pyroglutamate. Ref.5Molecular mass is 997.5 Da from positions 590 - 596. Determined by MALDI. Monophosphorylated. Ref.8Molecular mass is 3042 Da from positions 584 - 609. Determined by MALDI. Non-phosphorylatecd. Val-597. Ref.8Molecular mass is 3122 Da from positions 584 - 609. Determined by MALDI. Monophosphorylatecd. Val-597. Ref.8Molecular mass is 3154 Da from positions 584 - 609. Determined by MALDI. Monophosphorylatecd. Met-597. Ref.8Molecular mass is 3170 Da from positions 584 - 609. Determined by MALDI. Monophosphorylatecd. Oxidized Met-597. Ref.8Molecular mass is 3202 Da from positions 584 - 609. Determined by MALDI. Diphosphorylatecd. Val-597. Ref.8Molecular mass is 3250 Da from positions 584 - 609. Determined by MALDI. Diphosphorylatecd. Oxidized Met-597. Ref.8Molecular mass is 1460.3±0.4 Da from positions 624 - 646. Determined by ESI. Pyroglutamate. Ref.7