Western Blot (WB)
(Western Blot showing CHK1 antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2).)
Mouse CHK1 Monoclonal Antibody | anti-CHK1 antibody
CHK1 antibody
WB: 1:500-1:2,000
Western Blot (WB)
(Western Blot showing CHK1 antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2).)
Western Blot (WB)
(Western Blot showing CHK1 antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.
Protein type: Kinase, protein; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.1; CAMK group; CAMKL family; CHK1 subfamily
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: nucleoplasm; extracellular space; centrosome; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; chromatin; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; G2/M transition DNA damage checkpoint; DNA replication; G2/M transition of mitotic cell cycle; DNA repair; response to DNA damage stimulus; regulation of cell proliferation
Research Articles on CHK1
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Product Notes
The CHK1 chek1 (Catalog #AAA533959) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CHK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:10,000 WB: 1:500-1:2,000. Researchers should empirically determine the suitability of the CHK1 chek1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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