Mouse Aminopeptidase N ELISA Kit | Anpep elisa kit

Mouse Aminopeptidase N ELISA Kit

Cat. Num. AAA763399

• Gene Names: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
• Reactivity: Mouse
• Synonyms: Aminopeptidase N; Mouse Aminopeptidase N ELISA Kit; Anpep/CD13/AAP/Aminopeptidase N//ANPEP/APN/GP150/LAP1/P150/PEPN; Anpep elisa kit

• Reactivity: Mouse
• Sequence Length: 967

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Anpep elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Anpep antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Anpep antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Anpep amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Anpep can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157266300
• NCBI GeneID: 290
• NCBI Accession #: NP_001141.2
• NCBI GenBank Nucleotide #: NM_001150.2
• Molecular Weight: 109,540 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl aminopeptidase, membrane
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN; CD13; LAP1; P150; PEPN; GP150
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• Protein Family: Aminopeptidase
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: APN; CD13; PEPN; AP-N; hAPN; AP-M
• UniProt Entry Name: AMPN_HUMAN

NCBI Description

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Other Amino Acids Metabolism - glutathione; Receptor, misc.; EC 3.4.11.2; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 15q25-q26

Cellular Component: cytosol; ER-Golgi intermediate compartment; external side of plasma membrane; extracellular space; integral to plasma membrane; lysosomal membrane; vesicle membrane

Molecular Function: aminopeptidase activity; metallopeptidase activity; peptide binding; receptor activity; viral receptor activity; zinc ion binding

Biological Process: angiogenesis; angiotensin maturation; aromatic compound metabolic process; cell differentiation; cellular protein metabolic process; entry of virus into host cell; peptide catabolic process