Mouse Ceruloplasmin ELISA Kit | CER elisa kit

Mouse Ceruloplasmin ELISA Kit

Cat. Num. AAA731398

• Gene Names: CP; CP-2
• Reactivity: Mouse
• Synonyms: Ceruloplasmin; Mouse Ceruloplasmin ELISA Kit; CER elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This CER ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CER. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CER elisa kit

Principle of the assay: CER ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CER antibody and an CER-HRP conjugate. The assay sample and buffer are incubated together with CER-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CER concentration since CER from samples and CER-HRP conjugate compete for the anti-CER antibody binding site. Since the number of sites is limited, as more sites are occupied by CER from the sample, fewer sites are left to bind CER-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CER concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CER elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 1731685
• NCBI GeneID: 1356
• Molecular Weight: 122,205 Da
• NCBI Official Full Name: ceruloplasmin, partial
• NCBI Official Synonym Full Names: ceruloplasmin (ferroxidase)
• NCBI Official Symbol: CP
• NCBI Official Synonym Symbols: CP-2
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: CP
• UniProt Entry Name: CERU_HUMAN

NCBI Description

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family.

Protein type: Oxidoreductase; Secreted, signal peptide; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1; Secreted

Chromosomal Location of Human Ortholog: 3q23-q25

Cellular Component: extracellular space; lysosomal membrane; extracellular region

Molecular Function: ferroxidase activity; copper ion binding; chaperone binding

Biological Process: cellular iron ion homeostasis; copper ion transport; transmembrane transport

Disease: Aceruloplasminemia