Rabbit Centromere Protein B ELISA Kit | CENPB elisa kit

Rabbit Centromere Protein B ELISA Kit

Cat. Num. AAA720745

• Reactivity: Rabbit
• Synonyms: Centromere Protein B; Rabbit Centromere Protein B ELISA Kit; CENPB elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CENPB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CENPB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CENPB elisa kit

Principle of the Assay: CENPB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CENPB antibody and an CENPB-HRP conjugate. The assay sample and buffer are incubated together with CENPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CENPB concentration since CENPB from samples and CENPB-HRP conjugate compete for the anti-CENPB antibody binding site. Since the number of sites is limited, as more sites are occupied by CENPB from the sample, fewer sites are left to bind CENPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CENPB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CENPB elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 119630907
• NCBI GeneID: 1059
• Molecular Weight: 65,171 Da
• NCBI Official Full Name: centromere protein B, 80kDa
• NCBI Official Synonym Full Names: centromere protein B, 80kDa
• NCBI Official Symbol: CENPB
• NCBI Protein Information: major centromere autoantigen B; CENP-B
• UniProt Protein Name: Major centromere autoantigen B
• Protein Family: Major centromere autoantigen
• UniProt Gene Name: CENPB
• UniProt Synonym Gene Names: CENP-B
• UniProt Entry Name: CENPB_HUMAN

NCBI Description

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: chromosome; nucleus; chromosome, pericentric region

Molecular Function: centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding

Biological Process: regulation of transcription, DNA-dependent