Porcine Centromere protein V (CENPV) ELISA Kit | CENPV elisa kit

Porcine Centromere protein V (CENPV) ELISA Kit

Cat. Num. AAA756392

• Gene Names: CENPV; p30; PRR6; CENP-V; 3110013H01Rik
• Reactivity: Porcine
• Synonyms: Centromere protein V (CENPV); Porcine Centromere protein V (CENPV) ELISA Kit; CENPV elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CENPV ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CENPV. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CENPV elisa kit

Principle of the assay: CENPV ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CENPV antibody and an CENPV-HRP conjugate. The assay sample and buffer are incubated together with CENPV-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CENPV concentration since CENPV from samples and CENPV-HRP conjugate compete for the anti-CENPV antibody binding site. Since the number of sites is limited, as more sites are occupied by CENPV from the sample, fewer sites are left to bind CENPV-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CENPV concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CENPV elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 56118234
• NCBI GeneID: 201161
• NCBI Accession #: NP_859067.2
• NCBI GenBank Nucleotide #: NM_181716.2
• Molecular Weight: 29,731 Da
• NCBI Official Full Name: centromere protein V
• NCBI Official Synonym Full Names: centromere protein V
• NCBI Official Symbol: CENPV
• NCBI Official Synonym Symbols: p30; PRR6; CENP-V; 3110013H01Rik
• NCBI Protein Information: centromere protein V; proline rich 6; nuclear protein p30; proline-rich protein 6
• UniProt Protein Name: Centromere protein V
• Protein Family: Centromere protein
• UniProt Gene Name: CENPV
• UniProt Synonym Gene Names: PRR6; CENP-V
• UniProt Entry Name: CENPV_HUMAN

Uniprot Description

CENPV: Required for distribution of pericentromeric heterochromatin in interphase nuclei and for centromere formation and organization, chromosome alignment and cytokinesis. Belongs to the gfa family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: kinetochore; microtubule cytoskeleton; nucleoplasm; cytoplasm; spindle midzone; nucleus

Molecular Function: carbon-sulfur lyase activity

Biological Process: positive regulation of cytokinesis; mitosis; metabolic process; cell division; ameboidal cell migration; centric heterochromatin formation; regulation of chromosome organization and biogenesis