Mouse Centromere Protein A ELISA Kit | CENPA elisa kit

Mouse Centromere Protein A ELISA Kit

Cat. Num. AAA25256

• Gene Names: CENPA; CenH3; CENP-A
• Reactivity: Mouse
• Synonyms: Centromere Protein A; Mouse Centromere Protein A ELISA Kit; CENPA elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse CENPA and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CENPA concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse CENPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CENPA and CENPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CENPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CENPA elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CENPA concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse CENPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CENPA and CENPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CENPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 12803733
• NCBI GeneID: 1058
• UniProt Accession #: P49450; Q53T74; Q9BVW2; D6W544
• Molecular Weight: 15,991 Da
• NCBI Official Full Name: CENPA protein
• NCBI Official Synonym Full Names: centromere protein A
• NCBI Official Symbol: CENPA
• NCBI Official Synonym Symbols: CenH3; CENP-A
• NCBI Protein Information: histone H3-like centromeric protein A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone
• UniProt Protein Name: Histone H3-like centromeric protein A
• Protein Family: Histone H3-like centromeric protein
• UniProt Gene Name: CENPA
• UniProt Synonym Gene Names: CENP-A
• UniProt Entry Name: CENPA_HUMAN

NCBI Description

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; nucleus; cytosol; condensed nuclear chromosome, pericentric region; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; viral reproduction; mitotic cell cycle