Rabbit anti-Human, Mouse XRCC4 Polyclonal Antibody | anti-XRCC4 antibody

XRCC4 (X-ray Repair Cross-complementing Protein 4, DNA Repair Protein XRCC4) (Biotin)

Cat. Num. AAA6398801

• Gene Names: XRCC4; SSMED
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XRCC4; Polyclonal Antibody; XRCC4 (X-ray Repair Cross-complementing Protein 4; DNA Repair Protein XRCC4) (Biotin); anti-XRCC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XRCC4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 336

• Applicable Applications for anti-XRCC4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human XRCC4, aa1-336 (NP_071801.1).
• Immunogen Sequence: MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(XRCC4 rabbit polyclonal antibody. Western Blot analysis of XRCC4 expression in mouse liver)

Western Blot (WB)

(Western Blot analysis of XRCC4 expression in transfected 293T cell line by XRCC4 polyclonal antibody. Lane 1: XRCC4 transfected lysate (38.3kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-XRCC4 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 12408647
• NCBI GeneID: 7518
• NCBI Accession #: NP_071801.1
• NCBI GenBank Nucleotide #: NM_022406.1
• UniProt Accession #: Q13426
• NCBI Official Full Name: DNA repair protein XRCC4 isoform 2
• NCBI Official Synonym Full Names: X-ray repair cross complementing 4
• NCBI Official Symbol: XRCC4
• NCBI Official Synonym Symbols: SSMED
• NCBI Protein Information: DNA repair protein XRCC4
• UniProt Protein Name: DNA repair protein XRCC4
• Protein Family: Xrcc4-like factor
• UniProt Gene Name: XRCC4
• UniProt Entry Name: XRCC4_HUMAN

NCBI Description

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]

Uniprot Description

XRCC4: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (XRCC6/XRCC5) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF. Widely expressed. Belongs to the XRCC4 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 5q14.2

Cellular Component: nucleoplasm; DNA ligase IV complex; centrosome; condensed chromosome; DNA-dependent protein kinase complex; cytosol; cell junction; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; ligase activity

Biological Process: pro-B cell differentiation; positive regulation of neurogenesis; central nervous system development; viral reproduction; immunoglobulin V(D)J recombination; in utero embryonic development; T cell differentiation in the thymus; isotype switching; DNA repair; double-strand break repair via nonhomologous end joining; positive regulation of fibroblast proliferation; double-strand break repair; DNA ligation during DNA repair; response to gamma radiation; negative regulation of neuron apoptosis; positive regulation of ligase activity; response to X-ray

Disease: Short Stature, Microcephaly, And Endocrine Dysfunction