Testing Data
(Fig-1: Detection of human VISTA in the CHO-K1/VISTA stable cell line by Flow Cytometry [Cell surface staining]. CHO-K1 cells (Green); CHO-K1/VISTA cells (Red).)
VISTA cell line
VISTA Stable Cell Line
Applications
Functional Assay, Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Synonyms
VISTA; VISTA Stable Cell Line; VSIR; B7-H5; PD-1H; SISP1; Functional Assay; 14-516; VISTA cell line
Form/Format
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO
Sequence
MGVPTALEAGSWRWGSLLFALFLAASLGPVAAFKVATPYSLYVC PEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHG GHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRV HGAMELQVQTGKDAPSNCVVYPSSSQDSENITAAALATGACIVGILCLPLILLLVYKQ RQAASNRRAQELVRMDSNIQGIENPGFEASPPAQGIPEAKVRHPLSYVAQRQPSESGR HLLSEPSTPLSPPGPGDVFFPSLDPVPDSPNFEVI
Sequence Length
311
Applicable Applications for VISTA cell line
Functional Assay, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS supplemented with 10% FBS and 1% Pen/Strep, plus 10 ug/ml of Blasticidin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth medium without Blasticidin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
Shipping Note
Product available only in the USA.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Testing Data
(Fig-1: Detection of human VISTA in the CHO-K1/VISTA stable cell line by Flow Cytometry [Cell surface staining]. CHO-K1 cells (Green); CHO-K1/VISTA cells (Red).)
Testing Data
(Fig-1: Detection of human VISTA in the CHO-K1/VISTA stable cell line by Flow Cytometry [Cell surface staining]. CHO-K1 cells (Green); CHO-K1/VISTA cells (Red).)
Related Product Information for VISTA cell line
VISTA Stable Cell Line is a stably transfected CHO-K1 cell line which expresses human V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA, also known as VSIR, B7-H5, PD-1H, and SISP1).
Product Categories/Family for VISTA cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
C10orf54
UniProt Synonym Gene Names
; Sisp-1
UniProt Entry Name
VISTA_HUMAN
Uniprot Description
VISTA: May stimulate MMP14-mediated MMP2 activation
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q22.1
Cellular Component: cell surface; intercellular junction; plasma membrane
Biological Process: cell-cell adhesion
Similar Products
Product Notes
The VISTA c10orf54 (Catalog #AAA669350) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VISTA can be used in a range of immunoassay formats including, but not limited to, Functional Assay, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Western Blot (WB). Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS supplemented with 10% FBS and 1% Pen/Strep, plus 10 ug/ml of Blasticidin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth medium without Blasticidin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Researchers should empirically determine the suitability of the VISTA c10orf54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGVPTALEAG SWRWGSLLFA LFLAASLGPV AAFKVATPYS LYVC PEGQNVTLTC RLLGPVDKGH DVTFYKTWYR SSRGEVQTCS ERRPIRNLTF QDLHLHHG GHQAANTSHD LAQRHGLESA SDHHGNFSIT MRNLTLLDSG LYCCLVVEIR HHHSEHRV HGAMELQVQT GKDAPSNCVV YPSSSQDSEN ITAAALATGA CIVGILCLPL ILLLVYKQ RQAASNRRAQ ELVRMDSNIQ GIENPGFEAS PPAQGIPEAK VRHPLSYVAQ RQPSESGR HLLSEPSTPL SPPGPGDVFF PSLDPVPDSP NFEVI. It is sometimes possible for the material contained within the vial of "VISTA, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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