Rabbit anti-Human STAT5A/B Polyclonal Antibody | anti-STAT5A/B antibody

STAT5A/B (Phospho-Ser725/730) Antibody

Cat. Num. AAA9402963

• Gene Names: Stat5a; STAT5; AA959963
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: STAT5A/B; Polyclonal Antibody; STAT5A/B (Phospho-Ser725/730) Antibody; Signal transducer and activator of transcription 5B; STA5B; STAT5; transcription factor STAT5B; anti-STAT5A/B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of STAT5A/B only when phosphorylated at serine 725/ serine 730.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 793

• Applicable Applications for anti-STAT5A/B antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B.
• Modification: Phospho-Ser725/730
• Target Name: STAT5A/B
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-STAT5A/B (Ser725/730) antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-STAT5A/B antibody

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Product Categories/Family for anti-STAT5A/B antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6755672
• NCBI GeneID: 20850
• NCBI Accession #: NP_035618.1
• NCBI GenBank Nucleotide #: NM_011488.3
• UniProt Accession #: P42230
• Molecular Weight: 90,831 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: Stat5a
• NCBI Official Synonym Symbols: STAT5; AA959963
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• UniProt Gene Name: Stat5a
• UniProt Synonym Gene Names: Mgf; Mpf
• UniProt Entry Name: STA5A_MOUSE

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; DNA-binding; Transcription factor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; protein phosphatase binding; transcription factor activity

Biological Process: mammary gland involution; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; female pregnancy; positive regulation of multicellular organism growth; 2-oxoglutarate metabolic process; sequestering of lipid; allantoin metabolic process; negative regulation of mast cell apoptosis; natural killer cell differentiation; regulation of steroid metabolic process; T cell homeostasis; Peyer's patch development; isoleucine metabolic process; transcription, DNA-dependent; valine metabolic process; development of secondary female sexual characteristics; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; negative regulation of apoptosis; transcription from RNA polymerase II promoter; lactation; response to peptide hormone stimulus; succinate metabolic process; peptidyl-tyrosine phosphorylation; oxaloacetate metabolic process; signal transduction; positive regulation of activated T cell proliferation; positive regulation of mitotic cell cycle; fatty acid metabolic process; positive regulation of natural killer cell differentiation; regulation of transcription, DNA-dependent; mammary gland development; luteinization; positive regulation of cell proliferation; development of secondary male sexual characteristics; creatinine metabolic process; positive regulation of gamma-delta T cell differentiation; regulation of cell adhesion; negative regulation of erythrocyte differentiation; cytokine and chemokine mediated signaling pathway; citrate metabolic process; regulation of multicellular organism growth; positive regulation of natural killer cell mediated cytotoxicity; creatine metabolic process; positive regulation of lymphocyte differentiation; positive regulation of B cell differentiation; positive regulation of inflammatory response