NSL1 cdna clone
NSL1
Gene Names
NSL1; DC8; MIS14; C1orf48
Synonyms
NSL1; C1orf48; DC8; MIS14; NSL1 cdna clone
Form/Format
Lyophilized
Sequence
Nucleotide Sequence: ATGGCGGGGTCTCCTGAGTTGGTGGTCCTTGACCCTCCATGGGACAAGGAGCTCGCGGCTGGCACAGAGAGCCAGGCCTTGGTCTCCGCCACTCCCCGAGAAGACTTTCGGGTGCGCTGCACCTCGAAGCGGGCTGTGACCGAAATGCTACAACTGTGCGGCCGCTTCGTGCAAAAGCTCGGGGACGCTCTGCCGGAGGAGATTCGGGAGCCCGCTCTGCGAGATGCGCAGTGGACTTTTGAATCAGCTGTGCAAGAGAATATCAGCATTAATGGGCAAGCATGGCAGGAAGCTTCAGATAATTGTTTTATGGATTCTGACATCAAAGTACTTGAAGATCAGTTTGATGAAATCATAGTAGATATAGCCACAAAACGTAAGCAGTATCCCAGAAAGATCCTGGAATGTGTCATCAAAACCATAAAAGCAAAACAAGAAATTCTGAAGCAGTACCACCCTGTTGTACATCCACTGGACCTAAAATATGACCCTGATCCAGCCCCTCATATGGAAAATTTGAAATGCAGAGGGGAAACAGTAGCAAAGGAGATCAGTGAAGCCATGAAGTCCTTGCCTGCATTAATTGAACAAGGAGAGGGATTTTCCCAAGTTCTCAGGATGCAGCCTGTTATCCACCTCCAGAGGATTCACCAAGAAGTCTTTTCCAGTTGTCATAGGAAACCAGATGCTAAACCTGAGAACTTTATAACACAGATAGAAACCACACCAACAGAGACTGCTTCCAGGAAAACCTCTGACATGGTACTGAAAAGAAAGCAAACTAAAGACTGCCCCCAGAGAAAATGGTATCCATTGCGGCCAAAGAAAATTAATCTTGATACATGA
Translation Sequence: MAGSPELVVL DPPWDKELAA GTESQALVSA TPREDFRVRC TSKRAVTEML QLCGRFVQKLGDALPEEIRE PALRDAQWTF ESAVQENISI NGQAWQEASD NCFMDSDIKV LEDQFDEIIVDIATKRKQYP RKILECVIKT IKAKQEILKQ YHPVVHPLDL KYDPDPAPHM ENLKCRGETVAKEISEAMKS LPALIEQGEG FSQVLRMQPV IHLQRIHQEV FSSCHRKPDA KPENFITQIETTPTETASRK TSDMVLKRKQ TKDCPQRKWY PLRPKKINLD T
Translation Sequence: MAGSPELVVL DPPWDKELAA GTESQALVSA TPREDFRVRC TSKRAVTEML QLCGRFVQKLGDALPEEIRE PALRDAQWTF ESAVQENISI NGQAWQEASD NCFMDSDIKV LEDQFDEIIVDIATKRKQYP RKILECVIKT IKAKQEILKQ YHPVVHPLDL KYDPDPAPHM ENLKCRGETVAKEISEAMKS LPALIEQGEG FSQVLRMQPV IHLQRIHQEV FSSCHRKPDA KPENFITQIETTPTETASRK TSDMVLKRKQ TKDCPQRKWY PLRPKKINLD T
Sequence Length
281
Species
Human
Chromosome Location
1q41
OMIM Reference Number
609174
cDNA Size
846bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Vector
(puc19-derived cloning vector)
Preparation Before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Preparation and Storage
Store the plasmid at -20 degree C.
Related Product Information for NSL1 cdna clone
NSL1 encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. Multiple transcript variants encoding different isoforms have been found for NSL1. There is a pseudogene of the 3' UTR region of this gene on chromosome X.
Product Categories/Family for NSL1 cdna clone
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kinetochore-associated protein NSL1 homolog isoform 1
NCBI Official Synonym Full Names
NSL1 component of MIS12 kinetochore complex
NCBI Official Symbol
NSL1
NCBI Official Synonym Symbols
DC8; MIS14; C1orf48
NCBI Protein Information
kinetochore-associated protein NSL1 homolog
UniProt Protein Name
Kinetochore-associated protein NSL1 homolog
Protein Family
UniProt Gene Name
NSL1
UniProt Synonym Gene Names
C1orf48
UniProt Entry Name
NSL1_HUMAN
NCBI Description
This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]
Uniprot Description
NSL1: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: nucleus; cytosol; MIS12/MIND type complex
Molecular Function: protein binding
Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
Research Articles on NSL1
Similar Products
Product Notes
The NSL1 nsl1 (Catalog #AAA200887) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Nucleotide Sequence: ATGGCGGGGT CTCCTGAGTT GGTGGTCCTT GACCCTCCAT GGGACAAGGA GCTCGCGGCT GGCACAGAGA GCCAGGCCTT GGTCTCCGCC ACTCCCCGAG AAGACTTTCG GGTGCGCTGC ACCTCGAAGC GGGCTGTGAC CGAAATGCTA CAACTGTGCG GCCGCTTCGT GCAAAAGCTC GGGGACGCTC TGCCGGAGGA GATTCGGGAG CCCGCTCTGC GAGATGCGCA GTGGACTTTT GAATCAGCTG TGCAAGAGAA TATCAGCATT AATGGGCAAG CATGGCAGGA AGCTTCAGAT AATTGTTTTA TGGATTCTGA CATCAAAGTA CTTGAAGATC AGTTTGATGA AATCATAGTA GATATAGCCA CAAAACGTAA GCAGTATCCC AGAAAGATCC TGGAATGTGT CATCAAAACC ATAAAAGCAA AACAAGAAAT TCTGAAGCAG TACCACCCTG TTGTACATCC ACTGGACCTA AAATATGACC CTGATCCAGC CCCTCATATG GAAAATTTGA AATGCAGAGG GGAAACAGTA GCAAAGGAGA TCAGTGAAGC CATGAAGTCC TTGCCTGCAT TAATTGAACA AGGAGAGGGA TTTTCCCAAG TTCTCAGGAT GCAGCCTGTT ATCCACCTCC AGAGGATTCA CCAAGAAGTC TTTTCCAGTT GTCATAGGAA ACCAGATGCT AAACCTGAGA ACTTTATAAC ACAGATAGAA ACCACACCAA CAGAGACTGC TTCCAGGAAA ACCTCTGACA TGGTACTGAA AAGAAAGCAA ACTAAAGACT GCCCCCAGAG AAAATGGTAT CCATTGCGGC CAAAGAAAAT TAATCTTGAT ACATGA Transl ation Sequence: MAGSPELVVL DPPWDKELAA GTESQALVSA TPREDFRVRC TSKRAVTEML QLCGRFVQKL GDALPEEIRE PALRDAQWTF ESAVQENISI NGQAWQEASD NCFMDSDIKV LEDQFDEIIV DIATKRKQYP RKILECVIKT IKAKQEILKQ YHPVVHPLDL KYDPDPAPHM ENLKCRGETV AKEISEAMKS LPALIEQGEG FSQVLRMQPV IHLQRIHQEV FSSCHRKPDA KPENFITQIE TTPTETASRK TSDMVLKRKQ TKDCPQRKWY PLRPKKINLD T. It is sometimes possible for the material contained within the vial of "NSL1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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