MTHFS cdna clone

MTHFS

Cat. Num. AAA200780

• Gene Names: MTHFS; NEDMEHM; HsT19268
• Synonyms: MTHFS; HsT19268; MTHFS cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCGGCGGCAGCGGTGAGCAGCGCCAAGCGGAGCCTGCGGGGAGAGCTGAAGCAGCGTCTGCGGGCGATGAGTGCCGAGGAGCGGCTACGCCAGTCCCGCGTACTGAGCCAGAAGGTGATTGCCCACAGTGAGTATCAAAAGTCCAAAAGAATTTCCATCTTTCTGAGCATGCAAGATGAAATTGAGACAGAAGAGATCATCAAGGACATTTTCCAACGAGGCAAAATCTGCTTCATCCCTCGGTACCGGTTCCAGAGCAATCACATGGATATGGTGAGAATAGAATCACCAGAGGAAATTTCTTTACTTCCCAAAACATCCTGGAATATCCCTCAGCCTGGTGAGGGTGATGTTCGGGAGGAGGCCTTGTCCACAGGGGGACTTGATCTCATCTTCATGCCAGGCCTTGGGTTTGACAAACATGGCAACCGACTGGGGAGGGGCAAGGGCTACTATGATGCCTATCTGAAGCGCTGTTTGCAGCATCAGGAAGTGAAGCCCTACACCCTGGCGTTGGCTTTCAAAGAACAGATTTGCCTCCAGGTCCCAGTGAATGAAAACGACATGAAGGTAGATGAAGTCCTTTACGAAGACTCGTCAACAGCTTAA; ; Translation Sequence: MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQDEIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVREEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQICLQVPVNENDM KVDEVLYEDS STA
• Sequence Length: 203

• Species: Human
• Chromosome Location: 15q25.1
• OMIM Reference Number: 604197
• cDNA Size: 612bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for MTHFS cdna clone

MTHFS an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5, 10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for MTHFS.

Product Categories/Family for MTHFS cdna clone

Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5453746
• NCBI GeneID: 10588
• NCBI Accession #: NP_006432.1
• NCBI GenBank Nucleotide #: NM_006441.3
• UniProt Accession #: P49914
• NCBI Official Full Name: 5-formyltetrahydrofolate cyclo-ligase isoform a
• NCBI Official Synonym Full Names: methenyltetrahydrofolate synthetase
• NCBI Official Symbol: MTHFS
• NCBI Official Synonym Symbols: NEDMEHM; HsT19268
• NCBI Protein Information: 5-formyltetrahydrofolate cyclo-ligase
• UniProt Protein Name: 5-formyltetrahydrofolate cyclo-ligase
• UniProt Gene Name: MTHFS
• UniProt Synonym Gene Names: MTHFS; Methenyl-THF synthetase
• UniProt Entry Name: MTHFS_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.