DNA2 cdna clone
DNA2 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
DNA2: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. Belongs to the DNA2/NAM7 helicase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 10q21.3-q22.1
Cellular Component: gamma DNA polymerase complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus
Molecular Function: 5'-3' DNA helicase activity; 5'-flap endonuclease activity; ATPase activity; DNA binding; DNA helicase activity; helicase activity; nuclease activity; protein binding; single-stranded DNA-dependent ATPase activity; site-specific endodeoxyribonuclease activity, specific for altered base
Biological Process: base-excision repair; DNA double-strand break processing; DNA replication; DNA replication checkpoint; DNA replication, Okazaki fragment processing; DNA replication, removal of RNA primer; DNA synthesis during DNA repair; mitochondrial DNA repair; mitochondrial DNA replication; positive regulation of DNA replication; strand displacement; telomere maintenance; telomere maintenance via recombination
Disease: Progressive External Ophthalmoplegia With Mitochondrial Dna Deletions, Autosomal Dominant, 6; Seckel Syndrome 8
Research Articles on DNA2
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Product Notes
The DNA2 dna2 (Catalog #AAA1274666) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: ATGGAGCAGC TGAACGAACT GGAGCTGCTG ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG CTGCCGGCGG AGCTATTTCA GAAGAAAGTG GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC ACAGGAATGG ATAACCGGTA CCTGGTGTTG GCAGTCAATA CTGTACAGAA CAAAGAGGGA AACTGTGAAA AGCGCCTGGT CATCACTGCT TCACAGTCAC TAGAAAATAA AGAACTATGC ATCCTTAGGA ATGACTGGTG TTCTGTTCCA GTAGAGCCAG GAGATATCAT TCATTTGGAG GGAGACTGCA CATCTGACAC TTGGATAATA GATAAAGATT TTGGATATTT GATTCTGTAT CCAGACATGC TGATTTCTGG CACCAGCATA GCCAGTAGTA TTCGATGTAT GAGAAGAGCT GTCCTGAGTG AAACTTTTAG GAGCTCTGAT CCAGCCACAC GCCAAATGCT AATTGGTACG GTTCTCCATG AGGTGTTTCA AAAAGCCATA AATAATAGCT TTGCCCCAGA AAAGCTACAA GAACTTGCTT TTCAAACAAT TCAAGAAATA AGACATTTGA AGGAAATGTA CCGCTTAAAT CTAAGTCAAG ATGAAATAAA ACAAGAAGTA GAGGACTATC TTCCTTCGTT TTGTAAATGG GCAGGAGATT TCATGCATAA AAACACTTCG ACTGACTTCC CTCAGATGCA GCTCTCTCTG CCAAGTGATA ATAGTAAGGA TAATTCAACA TGTAACATTG AAGTCGTGAA ACCAATGGAT ATTGAAGAAA GCATTTGGTC CCCTAGGTTT GGATTGAAAG GCAAAATAGA TGTTACAGTT GGTGTGAAAA TACATCGAGG GTATAAAACA AAATACAAGA TAATGCCGCT GGAACTTAAA ACTGGCAAAG AATCAAATTC TATTGAACAC CGTAGTCAGG TTGTTCTGTA CACTCTACTA AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT GGCTTGCTTC TCTACCTCAA GACTGGTCAG ATGTACCCTG TGCCTGCCAA CCATCTAGAT AAAAGAGAAT TATTAAAGCT AAGAAACCAG ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT CTCGTAAGAT TCAGAAGGTT CATCCAGCTA TCCAGCAATT TACAGAGCAA GAAATTTGCA GATCAAAGTC CATTAAATCC TTAG. It is sometimes possible for the material contained within the vial of "DNA2, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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