CBFB cdna clone

CBFB

Cat. Num. AAA200536

• Gene Names: CBFB; PEBP2B
• Synonyms: CBFB; PEBP2B; CBFB cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGCCGCGCG TCGTGCCCGA CCAGAGAAGC AAGTTCGAGA ACGAGGAGTT TTTTAGGAAG CTGAGCCGCG AGTGTGAGAT TAAGTACACG GGCTTCAGGG ACCGGCCCCA CGAGGAACGC CAGGCACGCT TCCAGAACGC CTGCCGCGAC GGCCGCTCGG AAATCGCTTT TGTGGCCACA GGAACCAATC TGTCTCTCCA GTTTTTTCCG GCCAGCTGGC AGGGAGAACA GCGACAAACA CCTAGCCGAG AGTATGTCGA CTTAGAAAGA GAAGCAGGCA AGGTATATTT GAAGGCTCCC ATGATTCTGA ATGGAGTCTG TGTTATCTGG AAAGGCTGGA TTGATCTCCA AAGACTGGAT GGTATGGGCT GTCTGGAGTT TGATGAGGAG CGAGCCCAGC AGGAGGATGC ATTAGCACAA CAGGCCTTTG AAGAGGCTCG GAGAAGGACA CGCGAATTTG AAGATAGAGA CAGGTCTCAT CGGGAGGAAA TGGAGGTGAG AGTTTCACAG CTGCTGGCAG TAACTGGCAA GAAGACAACA AGACCCTAG; ; Translation Sequence: MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT REFEDRDRSH REEMEVRVSQ LLAVTGKKTT RP
• Sequence Length: 182

• Species: Human
• Chromosome Location: 16q22.1
• OMIM Reference Number: 121360
• cDNA Size: 549bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for CBFB cdna clone

CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e. g., RuNX1) and osteogenesis (e. g., RuNX2). This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit.

Product Categories/Family for CBFB cdna clone

Epigenomics (Transcription & Translation)

NCBI and Uniprot Product Information

• NCBI GI #: 13124873
• NCBI GeneID: 865
• NCBI Accession #: NP_001746.1
• NCBI GenBank Nucleotide #: NM_001755.2
• UniProt Accession #: Q13951
• NCBI Official Full Name: core-binding factor subunit beta isoform 2
• NCBI Official Synonym Full Names: core-binding factor subunit beta
• NCBI Official Symbol: CBFB
• NCBI Official Synonym Symbols: PEBP2B
• NCBI Protein Information: core-binding factor subunit beta
• UniProt Protein Name: Core-binding factor subunit beta
• Protein Family: Core-binding factor
• UniProt Gene Name: CBFB
• UniProt Synonym Gene Names: CBF-beta; PEA2-beta; PEBP2-beta
• UniProt Entry Name: PEBB_HUMAN

NCBI Description

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CBFB: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1. A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. Belongs to the CBF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: osteoblast differentiation; transcription from RNA polymerase II promoter; lymphocyte differentiation; cell maturation; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter