CDKN2D / p19ink4d recombinant protein

Recombinant Human CDKN2D / p19ink4d Protein (GST tag)

Cat. Num. AAA2545486

• Gene Names: CDKN2D; p19; INK4D; p19-INK4D
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CDKN2D / p19ink4d; Recombinant Human CDKN2D / p19ink4d Protein (GST tag); INK4D; p19; p19-INK4D; CDKN2D / p19ink4d recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 166

• Application Notes: The recombinant human CDKN2D/GST chimera consists of 400 amino acids and has a predicted molecular mass of 44.9 kDa. It migrates as an approxiamtely 46 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CDKN2D / p19ink4d recombinant protein

Background: Cyclin-dependent kinase inhibitor 2D (also known as CDKN2D or p19ink4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. It is clearly shown that DNA repair is the main target of p19ink4d effect and that diminished apoptosis is a downstream event. Experiments has uncovered a role of p19INK4d as a regulator of DNA-damage-induced apoptosis and suggest that it protects cells from undergoing apoptosis by allowing a more efficient DNA repair. It has been demonstrated that p19INK4d expression enhances cell survival under genotoxic conditions. Previous work has shown that inactivation of the cyclin-dependent kinase inhibitor (CKI) p19 (Ink4d) leads to progressive hearing loss attributable to inappropriate DNA replication and subsequent apoptosis of hair cells. It may also involved in male reproductive function including testicular atrophy, alteration in serum follicle stimulating hormone, qualitative increase in germ cell apoptosis, and delayed kinetics of meiotic prophase markers. 

Description: A DNA sequence encoding the human CDKN2D (P55273) (Met 10Leu 166) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4502753
• NCBI GeneID: 1032
• NCBI Accession #: NP_001791.1
• NCBI GenBank Nucleotide #: NM_001800.3
• UniProt Accession #: P55273; Q13102; Q6FGE9
• Molecular Weight: 17,700 Da
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor D
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 2D
• NCBI Official Symbol: CDKN2D
• NCBI Official Synonym Symbols: p19; INK4D; p19-INK4D
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor D
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor D
• UniProt Gene Name: CDKN2D

NCBI Description

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

CDKN2D: Interacts strongly with CDK4 and CDK6 and inhibits them. Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein type: Cell cycle regulation; Inhibitor; Tumor suppressor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: autophagic cell death; cell cycle arrest; DNA synthesis during DNA repair; G1/S transition of mitotic cell cycle; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of phosphorylation; regulation of cyclin-dependent protein kinase activity; response to retinoic acid; response to UV; response to vitamin D