p19INK4D recombinant protein

p19INK4D recombinant protein

Cat. Num. AAA515234

• Gene Names: CDKN2D; p19; INK4D; p19-INK4D
• Applications: Western Blot
• Synonyms: p19INK4D; p19INK4D recombinant protein; CDKN2D; p19; INK4D; p19-INK4D

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 1416

• Applicable Applications for p19INK4D recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human p19INK4D was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for p19INK4D recombinant protein

Recombinant full-length human p19INK4D was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: p19INK4D protein is associated with CDK6 in several hematopoietic cell lines. It has been demonstrated that p19INK4D expression enhances cell survival under genotoxic conditions (1). By using p19INK4D-overexpressing cells, it has been demonstrated that p19INK4D expression correlates with the cellular resistance to UV treatment with increased DNA repair activity against UV-induced lesions. p19INK4D regulates protein network that integrate DNA repair, apoptosis and checkpoint mechanisms in order to maintain the genomic integrity (2).

Product Categories/Family for p19INK4D recombinant protein

Cell Cycle Proteins; Signaling Proteins - Cell Cycle Proteins

References

1. Guan, K.L. et al: Isolation and characterization of p19INK4d, a p16-related inhibitor specific to CDK6 and CDK4. Mol Biol Cell. 1996; 7(1): 57 -70.
2. Julieta, M. et al: Induction of p19INK4d in response to ultraviolet light improves DNA repair and confers resistance to apoptosis in neuroblastoma cells. Oncogene, 2005; 24: 4065 -4080

NCBI and Uniprot Product Information

• NCBI GI #: 39995074
• NCBI GeneID: 1032
• NCBI Accession #: NP_001791.1
• NCBI GenBank Nucleotide #: NM_001800
• UniProt Accession #: P55273; Q13102; Q6FGE9
• Molecular Weight: ~44 kDa
• NCBI Official Full Name: Homo sapiens cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) (CDKN2D), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
• NCBI Official Symbol: CDKN2D
• NCBI Official Synonym Symbols: p19; INK4D; p19-INK4D
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor D; CDK inhibitor p19INK4d; inhibitor of cyclin-dependent kinase 4d; cyclin-dependent kinase 4 inhibitor D p19; cell cycle inhibitor, Nur77 associating protein
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor D
• UniProt Gene Name: CDKN2D
• UniProt Entry Name: CDN2D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Interacts strongly with CDK4 and CDK6 and inhibits them. Ref.2 Ref.3

Subunit structure: Interacts with CDK6. Ref.10

Subcellular location: Nucleus. Cytoplasm Ref.10.

Sequence similarities: Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.Contains 4 ANK repeats.