p19 INK4d Recombinant Protein | CDKN2D recombinant protein

p19 INK4d Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8118679

• Gene Names: CDKN2D; p19; INK4D; p19-INK4D
• Purity: >90 % as determined by SDS-PAGE
• Synonyms: p19 INK4d; p19 INK4d Protein; Human; Recombinant (GST Tag); Human CDKN2D/p19ink4d Protein (GST Tag); cyclin-dependent kinase inhibitor 2D (p19; inhibits CDK4); INK4D Protein; p19 Protein; p19-INK4D Protein; CDKN2D recombinant protein

• Host: E Coli
• Purity/Purification: >90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 400aa
• Sequence: Met10-Leu166

• Species: Human
• Tag: N-GST
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the human CDKN2D (P55273) (Met 10Leu 166) was fused with the GST tag at the N-terminus
• Activity: Immobilized human GST-CDKN2D at 10ug/ml (100ul/well) can bind biotinylated human GST-CDK4 (Cat:10732-H09B). The EC50 of biotinylated human GST-CDK4 (Cat:10732-H09B) is 0.52-1.2ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Immobilized human GST-CDKN2D at 10 ug/ml (100 ul/well) can bind biotinylated human GST-CDK4 (Cat: 10732-H09B), The EC50 of biotinylated human GST-CDK4 (Cat: 10732-H09B) is 0.52-1.2 ug/ml.)

Related Product Information for CDKN2D recombinant protein

Cyclin-dependent kinase inhibitor 2D(also known as CDKN2D or p19ink4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. It is clearly shown that DNA repair is the main target of p19ink4d effect and that diminished apoptosis is a downstream event. Experiments has uncovered a role of p19INK4d as a regulator of DNA-damage-induced apoptosis and suggest that it protects cells from undergoing apoptosis by allowing a more efficient DNA repair. It has been demonstrated that p19INK4d expression enhances cell survival under genotoxic conditions. Previous work has shown that inactivation of the cyclin-dependent kinase inhibitor (CKI) p19(Ink4d) leads to progressive hearing loss attributable to inappropriate DNA replication and subsequent apoptosis of hair cells. It may also involved in male reproductive function including testicular atrophy, alteration in serum follicle stimulating hormone, qualitative increase in germ cell apoptosis, and delayed kinetics of meiotic prophase markers.

References

Buchold GM, et al. (2007) Mice lacking cyclin-dependent kinase inhibitor p19Ink4d show strain-specific effects on male reproduction. Mol Reprod Dev. 74 (8): 1008-20. Laine H, et al. (2007) p19(Ink4d) and p21(Cip1) collaborate to maintain the postmitotic state of auditory hair cells, their codeletion leading to DNA damage and p53-mediated apoptosis. J Neurosci. 27 (6): 1434-44.Ceruti JM, et al. (2005) Induction of p19INK4d in response to ultraviolet light improves DNA repair and confers resistance to apoptosis in neuroblastoma cells. Oncogene. 24 (25): 4065-80.

NCBI and Uniprot Product Information

• NCBI GI #: 4502753
• NCBI GeneID: 1032
• NCBI Accession #: NP_001791.1
• NCBI GenBank Nucleotide #: NM_001800.3
• UniProt Accession #: P55273; Q13102; Q6FGE9
• Molecular Weight: 17,700 Da
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor D
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
• NCBI Official Symbol: CDKN2D
• NCBI Official Synonym Symbols: p19; INK4D; p19-INK4D
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor D; CDK inhibitor p19INK4d; inhibitor of cyclin-dependent kinase 4d; cyclin-dependent kinase 4 inhibitor D p19; cell cycle inhibitor, Nur77 associating protein
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor D
• Protein Family: Cyclin-dependent kinase 4 inhibitor
• UniProt Gene Name: CDKN2D
• UniProt Entry Name: CDN2D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

CDKN2D: Interacts strongly with CDK4 and CDK6 and inhibits them. Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein type: Tumor suppressor; Inhibitor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: response to retinoic acid; negative regulation of caspase activity; negative regulation of cell proliferation; DNA synthesis during DNA repair; response to vitamin D; sensory perception of sound; autophagic cell death; negative regulation of phosphorylation; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of cell growth; cell cycle arrest; G1/S transition of mitotic cell cycle; response to UV