SDS-Page
CDK2AP1 recombinant protein
CDK2AP1, 1-115aa, Human, His tag, E. coli
Gene Names
CDK2AP1; DOC1; ST19; DORC1; doc-1; p12DOC-1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
CDK2AP1; 1-115aa; Human; His tag; E. coli; Cyclin-dependent kinase 2 associated protein 1; CDKAP1; DOC1; CDKA1; Cyclin-dependent kinase 2 associated protein 1 CDK2 A1; CDK2 associated protein 1; Cyclin dependent kinase 2 associated protein 1 Deleted in oral cancer 1; DOC 1; DOC 1 related protein; DOC 1R; DOC1R; Putative oral cancer suppressor.; CDK2AP1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 50% glycerol, 2mM DTT
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS
Sequence Length
115
Applicable Applications for CDK2AP1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for CDK2AP1 recombinant protein
CDK2AP1 is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. Also, interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes. Recombinant CDK2AP1 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for CDK2AP1 recombinant protein
References
Tsuji T., et al. (1998) J Biol Chem. 273 (12):6704-9.; Hu MG., et al. (2004) Cancer Res. 64 (2):490-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16.6kDa (152aa), confirmed by MALDI-TOF.
NCBI Official Full Name
cyclin-dependent kinase 2-associated protein 1 isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase 2 associated protein 1
NCBI Official Symbol
CDK2AP1
NCBI Official Synonym Symbols
DOC1; ST19; DORC1; doc-1; p12DOC-1
NCBI Protein Information
cyclin-dependent kinase 2-associated protein 1
UniProt Protein Name
Cyclin-dependent kinase 2-associated protein 1
Protein Family
UniProt Gene Name
CDK2AP1
UniProt Synonym Gene Names
CDKAP1; DOC1; CDK2-associated protein 1; DOC-1
NCBI Description
The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
Uniprot Description
specific inhibitor of the cell-cycle kinase CDK2.
Research Articles on CDK2AP1
Similar Products
Product Notes
The CDK2AP1 cdk2ap1 (Catalog #AAA203474) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's CDK2AP1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CDK2AP1 cdk2ap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS. It is sometimes possible for the material contained within the vial of "CDK2AP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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