Mouse Adrenocorticotropic Hormone (ACTH) CLIA Kit | ACTH clia kit

Mouse Adrenocorticotropic Hormone (ACTH) CLIA Kit

Cat. Num. AAA2707932

• Gene Names: Pomc; BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Adrenocorticotropic Hormone (ACTH); Mouse Adrenocorticotropic Hormone (ACTH) CLIA Kit; Adrenocorticotropic Hormone; Corticotropin; Adrenocorticotropin; ACTH clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH).; No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
• Sequence Length: 39

• Applicable Applications for ACTH clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma and other biological fluids
• Detection Range: 11.72-3000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.12pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH). A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Adrenocorticotropic Hormone (ACTH) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 191659
• NCBI GeneID: 18976
• NCBI Official Full Name: adrenocorticotropic hormone, partial
• NCBI Official Synonym Full Names: pro-opiomelanocortin-alpha
• NCBI Official Symbol: Pomc
• NCBI Official Synonym Symbols: BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
• NCBI Protein Information: pro-opiomelanocortin

NCBI Description

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]