Monkey Cyclin Dependent Kinase 2 ELISA Kit | CDK-2 elisa kit

Monkey Cyclin Dependent Kinase 2 ELISA Kit

Cat. Num. AAA734224

• Reactivity: Monkey
• Synonyms: Cyclin Dependent Kinase 2; Monkey Cyclin Dependent Kinase 2 ELISA Kit; CDK-2 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CDK-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CDK-2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDK-2 elisa kit

Principle of the assay: CDK-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDK-2 antibody and an CDK-2-HRP conjugate. The assay sample and buffer are incubated together with CDK-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDK-2 concentration since CDK-2 from samples and CDK-2-HRP conjugate compete for the anti-CDK-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDK-2 from the sample, fewer sites are left to bind CDK-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDK-2 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 41054836
• NCBI GeneID: 362817
• NCBI Accession #: NP_955795.1
• NCBI GenBank Nucleotide #: NM_199501.1
• Molecular Weight: – Da
• NCBI Official Full Name: cyclin-dependent kinase 2
• NCBI Official Synonym Full Names: cyclin dependent kinase 2
• NCBI Official Symbol: Cdk2
• NCBI Protein Information: cyclin-dependent kinase 2; cell division protein kinase 2; cyclin dependent kinase 2-alpha
• UniProt Protein Name: Cyclin-dependent kinase 2
• UniProt Gene Name: Cdk2
• UniProt Synonym Gene Names: Cdkn2
• UniProt Entry Name: CDK2_RAT

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Protein kinase, CMGC; EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Cellular Component: Y chromosome; Cajal body; transcription factor complex; chromosome, telomeric region; intracellular membrane-bound organelle; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; microtubule organizing center; condensed chromosome; cytosol; nucleus; endosome

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; protein complex binding; histone kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: lens development in camera-type eye; response to drug; mitosis; organ regeneration; response to cAMP; regulation of cell cycle; response to toxin; positive regulation of transcription, DNA-dependent; histone phosphorylation; DNA repair; protein amino acid phosphorylation; response to estradiol stimulus; peptidyl-serine phosphorylation; response to cadmium ion; response to ethanol; meiotic cell cycle; cellular response to insulin stimulus; cell division; Ras protein signal transduction; positive regulation of cell proliferation; response to electrical stimulus; potassium ion transport; positive regulation of DNA replication initiation; G1/S transition of mitotic cell cycle