Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25followed by Goat anti Mouse IgG:FITC and dual stained with Rat anti Dog CD4)
Mouse anti-Canine CD25 Monoclonal Antibody | anti-CD25 antibody
CD25 (IL-2R Alpha Chain) (AP)
Reactivity
Canine
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
CD25; Monoclonal Antibody; CD25 (IL-2R Alpha Chain) (AP); anti-CD25 antibody
Host
Mouse
Reactivity
Canine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
P4A10
Specificity
Recognizes canine CD25
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-CD25 antibody
Western Blot (WB)
Immunogen
Enriched and stimulated Canine T cells
Conjugate
AP
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25followed by Goat anti Mouse IgG:FITC and dual stained with Rat anti Dog CD4)
Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25followed by Goat anti Mouse IgG:FITC and dual stained with Rat anti Dog CD4)
Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:FITC (MCA5916F) and dual stained with Rat anti Dog CD4)
Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:FITC (MCA5916F) and dual stained with Rat anti Dog CD4)
Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:RPE and dual stained with Rat anti Dog CD4)
Testing Data
(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:RPE and dual stained with Rat anti Dog CD4)
Product Categories/Family for anti-CD25 antibody
References
1. Abrams, V.K., et al. (2010) A novel monoclonal antibody specific for canine CD25 (P4A10): Selection and evaluation of canine Tregs. Vet Immunol. Immunopathol. 135: 257-265 2. Finn, J.D. et al. (2010) Eradication of neutralizing antibodies to factor VIII in canine hemophilia A after liver gene therapy. Blood. 116: 5842-8. 1. Rissetto, K.C. et al. (2010) Cloning and expression of canine CD25 for validation of an anti-human CD25 antibody to compare T regulatory lymphocytes in healthy dogs and dogs with osteosarcoma Vet. Immuno. Immunopath. 135:137-145 2. Pinheiro, D. et al. (2011) Phenotypic and functional characterization of a CD4+ CD25high FOXP3high regulatory T-cell population in the dog. Immunology 132:111-122 3. Bauer, T.R. et al. (2009) Potential Large Animal Models for Gene Therapy of Human Genetic Diseases of Immune and Blood Cell Systems. ILAR J 50:168-186 4. Paoloni M et al. (2008) Translation of new cancer treatments from pet dogs to humans. Nat. Rev. Cancer. 8:147-156
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Product Notes
The CD25 (Catalog #AAA6247559) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD25 (IL-2R Alpha Chain) (AP) reacts with Canine and may cross-react with other species as described in the data sheet. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CD25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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