Human cadherin 2, type 1, N-cadherin (neuronal) ELISA Kit | CDH2 elisa kit

Human Neural -Cadherin, N-Cad ELISA Kit

Cat. Num. AAA703223

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human
• Synonyms: cadherin 2; type 1; N-cadherin (neuronal); Human Neural -Cadherin; N-Cad ELISA Kit; N-Cad ELISA kit; CD325; CDHN; CDw325; NCAD; N-cadherin 1; N-cadherin (neuronal) ; calcium-dependent adhesion protein; neuronal; neural cadherin; CDH2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human N-Cad. No significant cross-reactivity or interference between human N-Cad and analogues was observed.
• Sequence Length: 906

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml -400 ng/ml.
• Sensitivity: The minimum detectable dose of human N-Cad is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDH2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for N-Cad has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any N-Cad present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N-Cad is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N-Cad bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 14589889
• NCBI GeneID: 1000
• NCBI Accession #: NP_001783.2
• NCBI GenBank Nucleotide #: NM_001792.3
• UniProt Accession #: P19022; Q14923; Q8N173; B0YIY6
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin
• UniProt Entry Name: CADH2_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density