Typical Testing Data/Standard Curve (for reference only)
Monkey Cadherin, Neuronal ELISA Kit | CDHN elisa kit
Monkey Cadherin, Neuronal ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This CDHN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CDHN. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CDHN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDHN antibody and an CDHN-HRP conjugate. The assay sample and buffer are incubated together with CDHN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDHN concentration since CDHN from samples and CDHN-HRP conjugate compete for the anti-CDHN antibody binding site. Since the number of sites is limited, as more sites are occupied by CDHN from the sample, fewer sites are left to bind CDHN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDHN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]
Uniprot Description
CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.
Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 18q11.2
Cellular Component: focal adhesion; cell-cell adherens junction; lamellipodium; apical plasma membrane; plasma membrane; integral to membrane; synapse; fascia adherens; intercellular junction; catenin complex
Molecular Function: protein binding; gamma-catenin binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; alpha-catenin binding
Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; muscle cell differentiation; cell migration; glial cell differentiation; positive regulation of MAPKKK cascade; calcium-dependent cell-cell adhesion; striated muscle cell differentiation; positive regulation of muscle cell differentiation; blood vessel morphogenesis; cell adhesion; homophilic cell adhesion
Research Articles on CDHN
Similar Products
Product Notes
The Monkey CDHN cdh2 (Catalog #AAA742601) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742601 ELISA Kit recognizes Monkey CDHN. It is sometimes possible for the material contained within the vial of "Cadherin, Neuronal, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
