SDS-PAGE
E-Cadherin / CDH1 / E-cad / CD324 Recombinant Protein | E-cad recombinant protein
Recombinant Mouse E-Cadherin / CDH1 / E-cad / CD324 Protein (His tag)
Gene Names
Cdh1; Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649
Purity
> 85 % as determined by SDS-PAGE
Synonyms
E-Cadherin / CDH1 / E-cad / CD324; Recombinant Mouse E-Cadherin / CDH1 / E-cad / CD324 Protein (His tag); AA960649; Ecad; L-CAM; MGC107495; UVO; Um; E-cad recombinant protein
Host
Human Cells
Purity/Purification
> 85 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
884
Application Notes
The secreted recombinant mouse CDH1 (pro form) comprises 697 amino acids and has a calculated molecular mass of 78 kDa. As a result of different glycosylation, the apparent molecular mass of rm CDH1 is approximately 80-100 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Glu 24
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for E-cad recombinant protein
Background: Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type . E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Description: A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Met 1-Val 709) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Met 1-Val 709) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,256 Da
NCBI Official Full Name
cadherin-1 preproprotein
NCBI Official Synonym Full Names
cadherin 1
NCBI Official Symbol
Cdh1
NCBI Official Synonym Symbols
Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649
NCBI Protein Information
cadherin-1
UniProt Protein Name
Cadherin-1
UniProt Gene Name
Cdh1
UniProt Synonym Gene Names
E-cadherin
NCBI Description
This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8. [provided by RefSeq, Oct 2015]
Uniprot Description
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 ().
Research Articles on E-cad
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Product Notes
The E-cad cdh1 (Catalog #AAA2546855) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse CDH1 (pro form) comprises 697 amino acids and has a calculated molecular mass of 78 kDa. As a result of different glycosylation, the apparent molecular mass of rm CDH1 is approximately 80-100 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the E-cad cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-Cadherin / CDH1 / E-cad / CD324, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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