CDCP1 recombinant protein

Recombinant Mouse CDCP1 Protein (His tag)

Cat. Num. AAA2546653

• Gene Names: Cdcp1; AA409659; 9030022E12Rik; E030027H19Rik
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: CDCP1; Recombinant Mouse CDCP1 Protein (His tag); CDCP1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 833

• Application Notes: The recombinant mouse CDCP1 comprises 648 amino acids and has a predicted molecular mass of 73.3 kDa. The apparent molecular mass of the protein is approximately 94-100 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ser 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CDCP1 recombinant protein

Background: CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia identification and for immature hematopoietic stem cell subsets.

Description: A DNA sequence encoding the mouse CDCP1 (Q5U462) (Met1-Leu666) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 61098143
• NCBI GeneID: 109332
• NCBI Accession #: NP_598735.2
• NCBI GenBank Nucleotide #: NM_133974.3
• UniProt Accession #: Q5U462; Q810J0; Q921M9
• Molecular Weight: 92,726 Da
• NCBI Official Full Name: CUB domain-containing protein 1
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: Cdcp1
• NCBI Official Synonym Symbols: AA409659; 9030022E12Rik; E030027H19Rik
• NCBI Protein Information: CUB domain-containing protein 1
• UniProt Protein Name: CUB domain-containing protein 1
• Protein Family: CUB domain-containing protein
• UniProt Gene Name: Cdcp1

Uniprot Description

CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9|9 F4