CDCP1 / CD318 recombinant protein

Human CDCP1 / CD318 Protein (His Tag)

Cat. Num. AAA2545344

• Gene Names: CDCP1; CD318; TRASK; SIMA135
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CDCP1 / CD318; Human CDCP1 / CD318 Protein (His Tag); CDCP1; TRASK; CDCP1 / CD318 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 836

• Application Notes: The recombinant human CDCP1 consists of 325 amino acids and has a predicted molecular mass of 36.4 kDa.
• Predicted N Terminal: Phe 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CDCP1 / CD318 recombinant protein

Background: CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia identification and for immature hematopoietic stem cell subsets.

Description: A DNA sequence encoding the human CDCP1 (Q9H5V8-3) (Met1-Glu343) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 30410805
• NCBI GeneID: 64866
• NCBI Accession #: NP_073753.3
• NCBI GenBank Nucleotide #: NM_022842.4
• UniProt Accession #: Q9H5V8; Q49UB4; Q6NT71; Q6U9Y2; Q8WU91; Q96QU7; Q9H676; Q9H8C2
• Molecular Weight: 37,818 Da
• NCBI Official Full Name: CUB domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: CDCP1
• NCBI Official Synonym Symbols: CD318; TRASK; SIMA135
• NCBI Protein Information: CUB domain-containing protein 1
• UniProt Protein Name: CUB domain-containing protein 1
• UniProt Gene Name: CDCP1
• UniProt Synonym Gene Names: TRASK; SIMA135

NCBI Description

This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Molecular Function: protein binding