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Western Blot (WB) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD(Phospho-Ser200) Antibody.)

Rabbit MyoD Polyclonal Antibody | anti-MyoD antibody

MyoD (Phospho-Ser200) Antibody

Gene Names
MYOD1; PUM; MYF3; MYOD; bHLHc1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
MyoD; Polyclonal Antibody; MyoD (Phospho-Ser200) Antibody; MYF3; MYOD; MYOD1; anti-MyoD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
320
Applicable Applications for anti-MyoD antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:1000
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.
Modification
Phospho-Ser200
Target Name
MyoD
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD(Phospho-Ser200) Antibody.)

Western Blot (WB) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD(Phospho-Ser200) Antibody.)
Related Product Information for anti-MyoD antibody
MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the
Product Categories/Family for anti-MyoD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,501 Da
NCBI Official Full Name
myoblast determination protein 1
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
NCBI Protein Information
myoblast determination protein 1
UniProt Protein Name
Myoblast determination protein 1
Protein Family
UniProt Gene Name
MYOD1
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
UniProt Entry Name
MYOD1_HUMAN

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; Transcription factor; RNA splicing

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome

Research Articles on MyoD

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Product Notes

The MyoD myod1 (Catalog #AAA9402320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MyoD (Phospho-Ser200) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MyoD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the MyoD myod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MyoD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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