CDC37 / CDC37A recombinant protein

Recombinant Human CDC37 / CDC37A Protein (GST tag)

Cat. Num. AAA2546152

• Gene Names: CDC37; P50CDC37
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CDC37 / CDC37A; Recombinant Human CDC37 / CDC37A Protein (GST tag); P50CDC37; CDC37 / CDC37A recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol, pH 7.4
• Sequence Length: 378

• Application Notes: The recombinant human CDC37/GST chimera consists of 603 amino acids and has a predicted a molecular mass of 70.7 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for CDC37 / CDC37A recombinant protein

Background: CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

Description: A DNA sequence encoding the full length of human CDC37 (NP_008996.1) (Met 1-Val 378) was fused with the GST tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 5901922
• NCBI GeneID: 11140
• NCBI Accession #: NP_008996.1
• NCBI GenBank Nucleotide #: NM_007065.3
• UniProt Accession #: Q16543; Q53YA2
• Molecular Weight: 44,468 Da
• NCBI Official Full Name: hsp90 co-chaperone Cdc37
• NCBI Official Synonym Full Names: cell division cycle 37
• NCBI Official Symbol: CDC37
• NCBI Official Synonym Symbols: P50CDC37
• NCBI Protein Information: hsp90 co-chaperone Cdc37
• UniProt Protein Name: Hsp90 co-chaperone Cdc37
• UniProt Gene Name: CDC37
• UniProt Synonym Gene Names: CDC37A

NCBI Description

The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC37: a molecular chaperone that functions in cell signal transduction. Forms complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. Also interacts with androgen receptor. It is thought to play a critical role in directing Hsp90 to its target kinases.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol

Molecular Function: chaperone binding; heat shock protein binding; kinase binding; protein binding; protein-tyrosine kinase activity; unfolded protein binding

Biological Process: protein folding; protein stabilization; protein targeting; regulation of cyclin-dependent protein kinase activity