Cytidine Deaminase Recombinant Protein | CDA recombinant protein

Recombinant Cytidine Deaminase (CDA)

Cat. Num. AAA2029569

• Gene Names: CDA; CDD
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cytidine Deaminase; Recombinant Cytidine Deaminase (CDA); CDA recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; AQKRPACTL KPECVQQLLV CSQEAKKSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKPDGTYIVMTVQ ELLPSSFGPE DLQKTQ
• Sequence Length: 146

• Applicable Applications for CDA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 19.9kDa
• Accurate Molecular Mass (KD): 17kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln1053~Lys1259
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 11386157
• NCBI GeneID: 978
• NCBI Accession #: NP_001776.1
• NCBI GenBank Nucleotide #: NM_001785.2
• Molecular Weight: 16,185 Da
• NCBI Official Full Name: cytidine deaminase
• NCBI Official Synonym Full Names: cytidine deaminase
• NCBI Official Symbol: CDA
• NCBI Official Synonym Symbols: CDD
• NCBI Protein Information: cytidine deaminase
• UniProt Protein Name: Cytidine deaminase
• Protein Family: Colicin
• UniProt Gene Name: CDA
• UniProt Synonym Gene Names: CDD
• UniProt Entry Name: CDD_HUMAN

NCBI Description

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: Nucleotide Metabolism - pyrimidine; EC 3.5.4.5; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 1p36.2-p35

Cellular Component: extracellular region; cytosol

Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity

Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; cytidine deamination; pyrimidine nucleoside salvage; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization