SDS-PAGE
CD99L2 / MIC2L1 recombinant protein
Recombinant Mouse CD99L2 / MIC2L1 Protein (His tag)
Gene Names
Cd99l2; Xap89; Mic2l1; AW548191
Purity
> 98 % as determined by SDS-PAGE
Synonyms
CD99L2 / MIC2L1; Recombinant Mouse CD99L2 / MIC2L1 Protein (His tag); RP23-125F4.3; AW548191; Mic2l1; Xap89; Cd99l2; CD99L2 / MIC2L1 recombinant protein
Host
Human Cells
Purity/Purification
> 98 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
214
Application Notes
The secreted recombinant mouse CD99L2 comprises 150 amino acids and has a predicted molecular mass of 16.3 kDa. As a result of glycosylation, the apparent molecular mass of rmCD99L2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Asp 26
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for CD99L2 / MIC2L1 recombinant protein
Background: Mouse CD99 antigen-like protein 2, also known as MIC2-like protein 1, CD99L2 and MIC2L1, is a single-pass type I membrane protein which belongs to the CD99 family. CD99L2 is expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression of CD99L2 is seen in thymus. It is also expressed in E18 uterus and placenta. CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-alpha, where the need for PECAM-1 is known to be bypassed. CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane. CD99L2 may function as a homophilic adhesion molecule. It functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. CD99L2 does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.
Description: A DNA sequence encoding the mouse CD99L2 (NP_612182.1) extracellular domain (Met 1-Ala 164) was expressed, fused with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the mouse CD99L2 (NP_612182.1) extracellular domain (Met 1-Ala 164) was expressed, fused with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,813 Da
NCBI Official Full Name
CD99 antigen-like protein 2 isoform 2
NCBI Official Synonym Full Names
CD99 antigen-like 2
NCBI Official Symbol
Cd99l2
NCBI Official Synonym Symbols
Xap89; Mic2l1; AW548191
NCBI Protein Information
CD99 antigen-like protein 2
UniProt Protein Name
CD99 antigen-like protein 2
UniProt Gene Name
Cd99l2
UniProt Synonym Gene Names
Mic2l1
Uniprot Description
CD99L2: Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation. Belongs to the CD99 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: X|X A7.3
Cellular Component: focal adhesion
Research Articles on CD99L2 / MIC2L1
1. Mice lacking CD99L2 had a defective inflammatory response in the thioglycollate peritonitis model
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Product Notes
The CD99L2 / MIC2L1 cd99l2 (Catalog #AAA2546936) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse CD99L2 comprises 150 amino acids and has a predicted molecular mass of 16.3 kDa. As a result of glycosylation, the apparent molecular mass of rmCD99L2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CD99L2 / MIC2L1 cd99l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD99L2 / MIC2L1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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