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SDS-Page

Ephrin-A1 Active Protein | EFNA1 active protein

Ephrin-A1 Protein, Mouse, Recombinant (hFc Tag)

Gene Names
Cd99l2; Xap89; Mic2l1; AW548191
Purity
>85% as determined by SDS-PAGE
Synonyms
Ephrin-A1; Ephrin-A1 Protein; Mouse; Recombinant (hFc Tag); Mouse Ephrin-A1/EFNA1 Protein (Fc Tag); AI325262 Protein; B61 Protein; Efl1 Protein; Epl1 Protein; Eplg1 Protein; Lerk1 Protein; ephrin-A1; EFNA1 active protein
Ordering
For Research Use Only!
Host
HEK293 Cells
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser182
Species
Mouse
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2ug/ml (100uL/well) can bind mouse EphrinA1 with a linear range of 0.16-20ng/mL.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asp 19
Tag
C-human IgG1-Fc
Protein Construction
A DNA sequence encoding the mouse EFNA1 (NP_034237.3) without the pro peptide (Met 1-Ser 182) was fused with the Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

SDS-Page

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2 ug/ml (100 ul/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.)

Testing Data (Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2 ug/ml (100 ul/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.)
Related Product Information for EFNA1 active protein
Background: EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.
References
Deroanne C, et al. (2003) EphrinA1 inactivates integrin-mediated vascular smooth muscle cell spreading via the Rac/PAK pathway. J Cell Sci. 116(7): 1367-76.Ojima T, et al. (2006) EphrinA1 inhibits vascular endothelial growth factor-induced intracellular signaling and suppresses retinal neovascularization and blood-retinal barrier breakdown. Am J Pathol. 168(1): 331-9.Wu D, et al. (2004) Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma. Gynecol Oncol. 94(2): 312-9.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,813 Da
NCBI Official Full Name
CD99 antigen-like protein 2 isoform 2
NCBI Official Synonym Full Names
CD99 antigen-like 2
NCBI Official Symbol
Cd99l2
NCBI Official Synonym Symbols
Xap89; Mic2l1; AW548191
NCBI Protein Information
CD99 antigen-like protein 2
Protein Family

Research Articles on EFNA1

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Product Notes

The EFNA1 (Catalog #AAA8122852) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser18 2. It is sometimes possible for the material contained within the vial of "Ephrin-A1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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