Ephrin-A1 Active Protein | EFNA1 active protein

Ephrin-A1 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122852

• Gene Names: Cd99l2; Xap89; Mic2l1; AW548191
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Ephrin-A1; Ephrin-A1 Protein; Mouse; Recombinant (hFc Tag); Mouse Ephrin-A1/EFNA1 Protein (Fc Tag); AI325262 Protein; B61 Protein; Efl1 Protein; Epl1 Protein; Eplg1 Protein; Lerk1 Protein; ephrin-A1; EFNA1 active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser182

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2ug/ml (100uL/well) can bind mouse EphrinA1 with a linear range of 0.16-20ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 19
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse EFNA1 (NP_034237.3) without the pro peptide (Met 1-Ser 182) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2 ug/ml (100 ul/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.)

Related Product Information for EFNA1 active protein

Background: EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.

References

Deroanne C, et al. (2003) EphrinA1 inactivates integrin-mediated vascular smooth muscle cell spreading via the Rac/PAK pathway. J Cell Sci. 116(7): 1367-76.Ojima T, et al. (2006) EphrinA1 inhibits vascular endothelial growth factor-induced intracellular signaling and suppresses retinal neovascularization and blood-retinal barrier breakdown. Am J Pathol. 168(1): 331-9.Wu D, et al. (2004) Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma. Gynecol Oncol. 94(2): 312-9.

NCBI and Uniprot Product Information

• NCBI GI #: 21489963
• NCBI GeneID: 171486
• NCBI Accession #: NP_612182.1
• NCBI GenBank Nucleotide #: NM_138309.3
• Molecular Weight: 22,813 Da
• NCBI Official Full Name: CD99 antigen-like protein 2 isoform 2
• NCBI Official Synonym Full Names: CD99 antigen-like 2
• NCBI Official Symbol: Cd99l2
• NCBI Official Synonym Symbols: Xap89; Mic2l1; AW548191
• NCBI Protein Information: CD99 antigen-like protein 2
• Protein Family: Ephrin