CD99L2 recombinant protein

CD99L2 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122799

• Gene Names: Cd99l2; Xap89; Mic2l1; AW548191
• Purity: >94% as determined by SDS-PAGE
• Synonyms: CD99L2; CD99L2 Protein; Mouse; Recombinant (hFc Tag); Mouse CD99L2/MIC2L1 Protein (Fc Tag); AW548191 Protein; Mic2l1 Protein; Xap89 Protein; CD99 molecule-like 2; CD99L2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala164

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 26
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse CD99L2 (NP_612182.1) extracellular domain (Met 1-Ala 164) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD99L2 recombinant protein

Background: CD99 antigen-like protein 2, also known as MIC2-like protein 1, CD99L2 and MIC2L1, is a single-pass type I membrane protein which belongs to theCD99 family. CD99L2 is expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression of CD99L2 is seen in thymus. It is also expressed in E18 uterus and placenta. CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-alpha, where the need for PECAM-1 is known to be bypassed. CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane. CD99L2 may function as a homophilic adhesion molecule. It functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. CD99L2 does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.

References

Suh, YH. et al., 2003, Gene. 307: 63-76. Park,S.H. Gene 2005, 353 (2):177-88. Schenkel, AR et al., 2007, Cell Commun Adhes. 14 (5):227-37. Bixel, MG. et al., 2010, Blood. 116 (7):1172-84.

NCBI and Uniprot Product Information

• NCBI GI #: 21489963
• NCBI GeneID: 171486
• NCBI Accession #: NP_612182.1
• NCBI GenBank Nucleotide #: NM_138309.3
• Molecular Weight: 22,813 Da
• NCBI Official Full Name: CD99 antigen-like protein 2 isoform 2
• NCBI Official Synonym Full Names: CD99 antigen-like 2
• NCBI Official Symbol: Cd99l2
• NCBI Official Synonym Symbols: Xap89; Mic2l1; AW548191
• NCBI Protein Information: CD99 antigen-like protein 2
• Protein Family: CD99 antigen-like protein