CD8B recombinant protein

CD8B, human recombinant

Cat. Num. AAA844561

• Gene Names: CD8B; LY3; P37; LEU2; LYT3; CD8B1
• Applications: SDS-Page
• Purity: >=80%
• Synonyms: CD8B; human recombinant; T-cell surface glycoprotein CD8 beta chain isoform 5 precursor; CD8B1; LEU2; LY3; LYT3; P37; CD8B recombinant protein

• Host: E Coli
• Purity/Purification: >=80%
• Form/Format: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea and 10% glycerol.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSLQQTPAY IKVQTNKMVM LSCEAKISLS NMRIYWLRQR QAPSSDSHHE FLALWDSAKG TIHGEEVEQE KIAVFRDASR FILNLTSVKP EDSGIYFCMI VGSPELTFGK GTQLSVVDFL PTTAQPTKKS TLKKRVCRLP RPETQKGPLC SP
• Sequence Length: 198

• Applicable Applications for CD8B recombinant protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CD8B recombinant protein

Background: CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Recombinant human CD8B protein, fused to His-tag at N-terminus, was expressed in E.coli.

Product Categories/Family for CD8B recombinant protein

Cytokines; Growth Factors & Hormones; CDsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth F

NCBI and Uniprot Product Information

• NCBI GI #: 296010933
• NCBI GeneID: 926
• NCBI Accession #: NP_001171571.1
• NCBI GenBank Nucleotide #: NM_001178100.1
• UniProt Accession #: P10966; P14860; P14861; Q15980; Q496E0; Q496E1; Q496E2; Q9UDB4; Q9UDB5; Q9UDB6; Q9UDB7; Q9UDB8
• Molecular Weight: 19.2 kDa (172 aa, 22-170 aa + His tag), confirmed by MALDI-TOF.
• NCBI Official Full Name: T-cell surface glycoprotein CD8 beta chain isoform 6
• NCBI Official Synonym Full Names: CD8b molecule
• NCBI Official Symbol: CD8B
• NCBI Official Synonym Symbols: LY3; P37; LEU2; LYT3; CD8B1
• NCBI Protein Information: T-cell surface glycoprotein CD8 beta chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 beta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8B
• UniProt Synonym Gene Names: CD8B1
• UniProt Entry Name: CD8B_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]

Uniprot Description

CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: T cell receptor complex; early endosome membrane; integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; MHC class I protein binding; coreceptor activity

Biological Process: regulation of immune response; T cell activation; viral reproduction; immune response; regulation of defense response to virus by virus; transmembrane receptor protein tyrosine kinase signaling pathway