CD8A & CD8B Heterodimer Recombinant Protein | CD8A&CD8B recombinant protein

CD8A & CD8B Heterodimer Protein, Mouse, Recombinant (DYKDDDDK & His Tag)

Cat. Num. AAA8123627

• Gene Names: Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD8A & CD8B Heterodimer; CD8A & CD8B Heterodimer Protein; Mouse; Recombinant (DYKDDDDK & His Tag); Mouse CD8A & CD8B Heterodimer Protein; CD8A&CD8B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr196&Met1-Thr175

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 28 & Leu 22
• Tag: C-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met1-Tyr196) of mouse CD8A (NP_001074579.1) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Thr175) of mouse CD8B (NP_033988.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD8A&CD8B heterodimer was purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 124487437
• NCBI GeneID: 12525
• NCBI Accession #: NP_001074579.1; NP_033988.1
• NCBI GenBank Nucleotide #: NM_001081110.2
• UniProt Accession #: P01731
• Molecular Weight: 27456 MW
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8 antigen, alpha chain
• NCBI Official Symbol: Cd8a
• NCBI Official Synonym Symbols: Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• UniProt Gene Name: Cd8a
• UniProt Synonym Gene Names: Lyt-2; Lyt2

Uniprot Description

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.