Rabbit anti-Human GSG1L Polyclonal Antibody | anti-GSG1L antibody

GSG1L, CT (GSG1L, Germ cell-specific gene 1-like protein) (Azide free) (HRP)

Cat. Num. AAA6304650

• Gene Names: GSG1L; PRO19651
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GSG1L; Polyclonal Antibody; CT (GSG1L; Germ cell-specific gene 1-like protein) (Azide free) (HRP); Germ cell-specific gene 1-like protein; anti-GSG1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GSG1L antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GSG1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human GSG1L.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GSG1L Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GSG1L antibody detected the GSG1L protein (arrow).)

Immunohistochemistry (IHC)

(GSG1L Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSG1L Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-GSG1L antibody

Antibodies; Proteins

References

Rual, J.F., et al. Genome Res. 14 (10B), 2128-2135 (2004): Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 157951718
• NCBI GeneID: 146395
• NCBI Accession #: NP_001103233.1; NP_653276.1
• NCBI GenBank Nucleotide #: NM_001109763.1
• UniProt Accession #: Q6UXU4; Q7Z6F8; Q8TB81
• Molecular Weight: 36,774 Da
• NCBI Official Full Name: germ cell-specific gene 1-like protein isoform 1
• NCBI Official Synonym Full Names: GSG1-like
• NCBI Official Symbol: GSG1L
• NCBI Official Synonym Symbols: PRO19651
• NCBI Protein Information: germ cell-specific gene 1-like protein; KTSR5831; GSG1-like protein
• UniProt Protein Name: Germ cell-specific gene 1-like protein
• Protein Family: Germ cell-specific gene 1-like protein
• UniProt Gene Name: GSG1L
• UniProt Synonym Gene Names: GSG1-like protein
• UniProt Entry Name: GSG1L_HUMAN

Uniprot Description

Function: As a component of the inner core of AMPAR complex, modifies AMPA receptor (AMPAR) gating

By similarity.

Subunit structure: Component of the inner core of AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing

By similarity.

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse

By similarity.

Sequence similarities: Belongs to the GSG1 family.