Typical Testing Data/Standard Curve (for reference only)
Chicken GATA3 ELISA Kit | GATA3 elisa kit
Chicken GATA3 (GATA Binding Protein 3) ELISA Kit
Reactivity
Chicken
Synonyms
GATA3; Chicken GATA3 (GATA Binding Protein 3) ELISA Kit; GATA3 elisa kit
Reactivity
Chicken
Specificity
This kit recognizes natural and recombinant Chicken GATA3. No significant cross-reactivity or interference between Chicken GATA3 and analogues was observed.
Sequence Length
444
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GATA3 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken GATA3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GATA3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GATA3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GATA3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GATA3. You can calculate the concentration of GATA3 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GATA3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GATA3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GATA3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GATA3. You can calculate the concentration of GATA3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,197 Da
NCBI Official Full Name
GATA-binding factor 3
NCBI Official Synonym Full Names
GATA binding protein 3
NCBI Official Symbol
GATA3
NCBI Protein Information
GATA-binding factor 3; GATA-3; NF-E1; transcription factor NF-E1c
UniProt Protein Name
GATA-binding factor 3
Protein Family
UniProt Gene Name
GATA3
UniProt Synonym Gene Names
GATA-3
UniProt Entry Name
GATA3_CHICK
Uniprot Description
Transcriptional activator which probably serves as a general switch factor for cell-specific development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of genes.
Research Articles on GATA3
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Product Notes
The Chicken GATA3 gata3 (Catalog #AAA2503604) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503604 ELISA Kit recognizes Chicken GATA3. It is sometimes possible for the material contained within the vial of "GATA3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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